{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:11:07Z","timestamp":1772118667666,"version":"3.50.1"},"reference-count":90,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T00:00:00Z","timestamp":1708214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:italic>TRPS1<\/jats:italic>\n                      serves as the causative gene for tricho\u2010rhino phalangeal syndrome, known for its craniofacial and skeletal abnormalities. The\n                      <jats:italic>Trps1<\/jats:italic>\n                      gene encodes a protein that represses Wnt signaling through strong interactions with Wnt signaling inhibitors. The identification of genomic cis\u2010acting regulatory sequences governing\n                      <jats:italic>Trps1<\/jats:italic>\n                      expression is crucial for understanding its role in embryogenesis. Nevertheless, to date, no investigations have been conducted concerning these aspects of\n                      <jats:italic>Trps1<\/jats:italic>\n                      .\u00a0To identify deeply conserved noncoding elements (CNEs) within the\n                      <jats:italic>Trps1<\/jats:italic>\n                      locus, we employed a comparative genomics approach, utilizing slowly evolving fish such as coelacanth and spotted gar. These analyses resulted in the identification of eight CNEs in the intronic region of the\n                      <jats:italic>Trps1<\/jats:italic>\n                      gene. Functional characterization of these CNEs in zebrafish revealed their regulatory potential in various tissues, including pectoral fins, heart, and pharyngeal arches. RNA in\u2010situ hybridization experiments revealed concordance between the reporter expression pattern induced by the identified set of CNEs and the spatial expression pattern of the\n                      <jats:italic>trps1<\/jats:italic>\n                      gene in zebrafish. Comparative in vivo data from zebrafish and mice for CNE7\/hs919 revealed conserved functions of these enhancers. Each of these eight CNEs was further investigated in cell line\u2010based reporter assays, revealing their repressive potential. Taken together, in vivo and in vitro assays suggest a context\u2010dependent dual functionality for the identified set of\n                      <jats:italic>Trps1<\/jats:italic>\n                      \u2010associated CNE enhancers.\u00a0This functionally characterized set of CNE\u2010enhancers will contribute to a more comprehensive understanding of the developmental roles of\n                      <jats:italic>Trps1<\/jats:italic>\n                      and can aid in the identification of noncoding DNA variants associated with human diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jez.b.23246","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T02:53:06Z","timestamp":1708311186000},"page":"85-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cis\u2010regulatory control of mammalian\n                    <i>Trps1<\/i>\n                    gene expression"],"prefix":"10.1002","volume":"342","author":[{"given":"Muhammad","family":"Abrar","sequence":"first","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahid","family":"Ali","sequence":"additional","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Organismal Biology and Anatomy The University of Chicago Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irfan","family":"Hussain","sequence":"additional","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Center of Regenerative Medicine and Stem Cells Research Aga Khan University Hospital Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hizran","family":"Khatoon","sequence":"additional","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Batool","sequence":"additional","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shakira","family":"Ghazanfar","sequence":"additional","affiliation":[{"name":"National Institute for Genomics Advanced Biotechnology National Agriculture Research Centre (NARC) Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dylan","family":"Corcoran","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Development University of Minnesota Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Development University of Minnesota Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4556-8129","authenticated-orcid":false,"given":"Amir Ali","family":"Abbasi","sequence":"additional","affiliation":[{"name":"National Center for Bioinformatics, Program of Comparative and Evolutionary Genomics, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad 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