{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:07:34Z","timestamp":1790878054800,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Exp Zool Pt B"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The evolutionary change of the trigeminal nerve\u2010innervation pattern is essential to reveal the mechanism underlying jaw acquisition. However, the homology of the branches between gnathostomes (jawed vertebrates) and cyclostomes (living jawless vertebrates) remains unclear. In this study, we focused on a subbranch called the ramus subpharyngeus, which belongs to the second branch of the lamprey trigeminal nerve and projects to the lower lip, investigating whether it contains motor components. To visualize motor fibers, we performed acetylcholinesterase (AChE) staining, a histochemical method that visualizes intrinsic activities of the catabolic enzymes produced by motor neurons and muscle fibers. As a result, we found AChE staining signals that correspond to the innervation course of the ramus subpharyngeus. To confirm that these signals in this region do not mark the motoneuronal somata nor muscle fibers, we conducted gene expression analysis by in situ hybridization. The results support that the signals mark the motor fibers. Based on these results, we propose that the lamprey oral apparatus is chiefly controlled by the second (i.e., premandibular) branch of the trigeminal nerve and further suggest that a drastic reorganization of the anterior craniofacial region occurred during the acquisition of the vertebrate jaw.<\/jats:p>","DOI":"10.1002\/jez.b.23316","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:10:38Z","timestamp":1751587838000},"page":"341-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Whole\u2010Mount Acetylcholinesterase Staining Reveals Unique Motor Innervation of the Lamprey Oral Region: With Special Reference to the Evolutionary Origin of the Vertebrate Jaw"],"prefix":"10.1002","volume":"344","author":[{"given":"Motoki","family":"Tamura","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3742-183X","authenticated-orcid":false,"given":"Daichi G.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Institute of Life and Environmental Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"131","article-title":"The Peripheral Distribution of the Cranial Nerves of Ammocoetes","volume":"33","author":"Alcock R.","year":"1898","journal-title":"Journal of Anatomy and Physiology"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051940102"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(93)90314-U"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2011.00465.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6395.2010.00472.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20240"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.117.2.409"},{"key":"e_1_2_8_10_1","first-page":"1","article-title":"Recherches sur le d\u00e9veloppement de Lampetra fluviatilis L.: Contribution \u00c0 L'\u00e9tude De La Cephalog\u00e9n\u00e8se Des Vert\u00e9br\u00e9s","volume":"55","author":"Damas H.","year":"1944","journal-title":"Arch Biol (Paris)"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2008.03.004"},{"key":"e_1_2_8_12_1","volume-title":"The Cranial Muscles of Vertebrates","author":"Edgeworth F. H.","year":"1935"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/361129a0"},{"issue":"4","key":"e_1_2_8_14_1","first-page":"465","article-title":"Origin of Vertebrates, Deduced From the Study of Ammocoetes: Parts V\u2013VIII","volume":"34","author":"Gaskell W. H.","year":"1900","journal-title":"Journal of Anatomy and Physiology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000088128"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80778-3"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.82144"},{"key":"e_1_2_8_18_1","volume-title":"The Biology of Lampreys","author":"Hardisty M. W.","year":"1971"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20193"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12687-4"},{"key":"e_1_2_8_21_1","unstructured":"Janvier P.(1985). Les Cephalaspides du Spitsberg. Cahiers de Pal\u00e9ontologie Section Vert\u00e9br\u00e9s: \u00c9ditions du Centre National de la Recherche Scientifique."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198540472.001.0001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6395.1948.tb00030.x"},{"key":"e_1_2_8_24_1","first-page":"149","article-title":"The Cranial Nerve Components of Petromyzon","volume":"34","author":"Johnston J. B.","year":"1905","journal-title":"Morphologisches Jahrbuch"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04506-x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.657"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/12.3.219"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.05.080"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icn048"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902640402"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006595"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.23.1053"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.22.1"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2011.00523.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21011"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970811)384:4<483::AID-CNE1>3.0.CO;2-Z"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.10.029"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.20009"},{"key":"e_1_2_8_39_1","first-page":"285","article-title":"Chemoarchitectonics of the Forebrain of the Hagfish, Eptatretus burgeri","volume":"22","author":"Kusunoki T.","year":"1981","journal-title":"Journal fur Hirnforschung"},{"issue":"1","key":"e_1_2_8_40_1","first-page":"109","article-title":"Chemoarchitectonics of the Brain Stem of the Hagfish, Eptatretus burgeri, With Special Reference to the Primordial Cerebellum","volume":"23","author":"Kusunoki T.","year":"1982","journal-title":"Journal fur Hirnforschung"},{"issue":"1","key":"e_1_2_8_41_1","first-page":"13","article-title":"The Chemoarchitectonics of the Shark Brain","volume":"14","author":"Kusunoki T.","year":"1973","journal-title":"Journal fur Hirnforschung"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6395.1949.tb00509.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1996.tb01658.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.25.990"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04691"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12187"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12627"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.18.3521"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00473.x"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11794"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13723"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017396"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-188"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.2009.00092.x"},{"key":"e_1_2_8_56_1","volume-title":"The Vertebrate Body, 6th edition","author":"Romer A. S.","year":"1986"},{"key":"e_1_2_8_57_1","first-page":"289","volume-title":"The Skull Volume 1: Development","author":"Rose C. S.","year":"1993"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068310"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20182"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40851-016-0046-3"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-99338-1"},{"key":"e_1_2_8_62_1","first-page":"109","article-title":"Normal Stages of Development in the Lamprey, Lampetra reissued (Dybowski)","volume":"5","author":"Tahara Y.","year":"1988","journal-title":"Zoological Science"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40851-023-00222-9"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.8.2569"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.9.2397"},{"key":"e_1_2_8_66_1","first-page":"470","article-title":"Die Schleimknorpeligen Bestandteile im Kopfskelett von Ammocoetes","volume":"133","author":"Tretjakoff D.","year":"1929","journal-title":"Zeitschrift fur wissenschartliche Zoologie"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.06.008"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5060-06.2007"},{"key":"e_1_2_8_69_1","first-page":"1","article-title":"The Downtonian and Devonian Vertebrates of Spitsbergen. IX, Morphologic and Systematic Studies of the Spitsbergen Cephalaspids. Results of Th. Vogt's Expedition 1928 and the English\u2010Norwegian\u2010Swedish Expedition in 1939","volume":"97","author":"W\u00e4ngsj\u00f6 G.","year":"1952","journal-title":"Norsk Polarinstitutt, Skrifter"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.243"}],"container-title":["Journal of Experimental Zoology Part B: Molecular and Developmental Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jez.b.23316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T10:56:13Z","timestamp":1767005773000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jez.b.23316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":69,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/jez.b.23316"],"URL":"https:\/\/doi.org\/10.1002\/jez.b.23316","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JEZ.B.23316\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JEZ.B.23316\/v1\/decision1","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.04.10.648078","asserted-by":"object"}]},"ISSN":["1552-5007","1552-5015"],"issn-type":[{"value":"1552-5007","type":"print"},{"value":"1552-5015","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]},"assertion":[{"value":"2024-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}