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Diving beetles (Dytiscidae) are predaceous freshwater insects with strictly aquatic larvae and amphibious adults. Using RNA\u2010seq, we compared the expression of odorant receptors (ORs), gustatory receptors (GRs), ionotropic receptors (IRs), and odorant\u2010binding proteins (OBPs) in the cephalic appendages of larval and adult\n                    <jats:italic>Cybister lateralimarginalis<\/jats:italic>\n                    . Overall, larvae expressed fewer chemosensory genes than adults but larva\u2010specific genes displayed a unique expression pattern, not previously observed in any other holometabolous insect, with five larva\u2010specific ORs all having a close paralogue which is adult\u2010specific, 14 larva\u2010specific IRs all belonging to a single gene expansion in the IR tree, and no larva\u2010specific GR. Expression profiles across appendage types mirrored those in aerial insects, with ORs mainly in antennae, GRs in labial palps, \u201cAntennal class\u201d IRs in antennae, and \u201cDivergent class\u201d IRs in palps. This suggests that the land\u2010to\u2010freshwater transition in this lineage did not involve major changes in deployment of the major families of chemosensory genes among cephalic appendages. Notably, the expression of a substantial repertoire of ORs specifically in the antennae of the larva suggests that hydrophobic chemical cues are important for long\u2010range chemodetection in freshwater, contrary to prevailing views about constraints for chemosensation within a water medium.\n                  <\/jats:p>","DOI":"10.1002\/jezb.70007","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T04:36:58Z","timestamp":1765773418000},"page":"390-410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of Chemosensory Genes in Larval and Adult Cephalic Appendages in a Diving Beetle"],"prefix":"10.1002","volume":"346","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0764-4127","authenticated-orcid":false,"given":"Marie","family":"Merle","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, IRD, UMR \u00c9volution, G\u00e9nomes, 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