{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T09:47:04Z","timestamp":1778579224627,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior - Brazil","doi-asserted-by":"crossref","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JGH Open"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and aim<\/jats:title><jats:p>Human gut microbiota play an important role in metabolism and host physiology. Perturbations of the gut microbial communities lead to the development of various diseases such as inflammatory bowel disease, celiac disease, allergic diseases, and metabolic diseases. Crohn's disease is a chronic inflammatory bowel disease characterized by periods of remission and relapse. Several studies suggest that intestinal inflammation arises due to an abnormal response of the intestinal immune system to the fecal microbiota. The goal of the study was to evaluate the relative amount of four bacterial groups in fecal samples of Crohn's disease patients and their relation to the inflammatory activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied stool samples of 105 individuals, 54 with Crohn's disease and 51 as a control group. The DNA extracted from the stool samples was subjected to real\u2010time polymerase chain reaction (qPCR) for quantification of the Bacteroidetes phylum, class Bacilli, and Bifidobacteriaceae and Enterobacteriaceae families.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found a significant increase in Bacteroidetes in Crohn's disease samples when compared to the control group (14\u2009650 and 2060\u2009CFU\/ng DNA, respectively) (<jats:italic>P<\/jats:italic> = 0.014). On the other hand, we observed a significant reduction in Bacilli and Bifidobacteriaceae (13 and 58\u2009CFU\/ng DNA, respectively) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). In contrast, patients without any drug treatment presented an increase of Bacilli and Bifidobacteriaceae (102\u2009521 and 6235\u2009CFU\/ng DNA, respectively) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The commensal bacteria were decreased in fecal samples of participants with Crohn's disease when compared to the control group. There was no relation between the disease location and\/or disease activity with the microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgh3.12338","type":"journal-article","created":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T13:39:14Z","timestamp":1588340354000},"page":"784-789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The relationship between the commensal microbiota levels and Crohn's disease activity"],"prefix":"10.1002","volume":"4","author":[{"given":"Hagamenon","family":"de Alencar Junior","sequence":"first","affiliation":[{"name":"Department of Pathology Universidade Federal de S\u00e3o Paulo\u2014Paulista Medical School, UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-7515","authenticated-orcid":false,"given":"Ana Paula Ribeiro","family":"Paiotti","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Universidade Federal de S\u00e3o Paulo\u2014Paulista Medical School, UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto Bezerra","family":"de Ara\u00fajo Filho","sequence":"additional","affiliation":[{"name":"Division of Pediatric Gastroenterology Universidade Federal de S\u00e3o Paulo\u2014Paulista Medical School, UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celina Tizuko Fujiyama","family":"Oshima","sequence":"additional","affiliation":[{"name":"Department of Pathology Universidade Federal de S\u00e3o Paulo\u2014Paulista Medical School, UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sender Jankiel","family":"Miszputen","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Universidade Federal de S\u00e3o Paulo\u2014Paulista Medical School, UNIFESP  S\u00e3o Paulo 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