{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:45:11Z","timestamp":1773816311178,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T00:00:00Z","timestamp":1726617600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2022\u201004192"],"award-info":[{"award-number":["2022\u201004192"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009780","name":"Region Sk\u00e5ne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011077","name":"Sk\u00e5nes universitetssjukhus","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011077","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JGH Open"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Full\u2010thickness biopsies of the intestinal wall may be used to study and assess damage to the neurons of the enteric nervous system (ENS), that is, enteric neuropathy. The ENS is difficult to examine due to its localization deep in the intestinal wall and its organization with several connections in diverging directions. Histological sections used in clinical practice only visualize the sample in a two\u2010dimensional way. X\u2010ray phase\u2010contrast micro\u2010computed tomography (PC\u2010\u03bcCT) has shown potential to assess the cross\u2010sectional thickness and volume of the ENS in three dimensions (3D). The aim of this study was to explore the potential of PC\u2010\u03bcCT to evaluate its use to determine the size of the ENS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Full\u2010thickness biopsies of ileum obtained during surgery from five controls and six patients clinically diagnosed with enteric neuropathy and dysmotility were included. Punch biopsies of 1\u2009mm in diameter and 1\u2009cm in length, from an area containing myenteric plexus, were extracted from paraffin blocks, and scanned with synchrotron\u2010based PC\u2010\u03bcCT without any staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The microscopic volumetric structure of the neural tissue (consisting of both ganglia and fascicles) could be determined in all samples. The ratio of neural tissue volume\/total tissue volume was higher in controls than in patients with enteric neuropathy (<jats:italic>P<\/jats:italic>\u2009=\u20090.013). The patient with the longest disease duration had the lowest ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The assessment of neural tissue can be performed in an objective, standardized way, to ensure reproducibility and comparison under physiological and pathological conditions. Further evaluation is needed to examine the role of this method in the diagnosis of enteric neuropathy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgh3.70027","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T07:31:20Z","timestamp":1726644680000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Volumetric changes of the enteric nervous system under physiological and pathological conditions measured using x\u2010ray phase\u2010contrast tomography"],"prefix":"10.1002","volume":"8","author":[{"given":"Niccol\u00f2","family":"Peruzzi","sequence":"first","affiliation":[{"name":"Medical Radiation Physics, Department of Clinical Sciences Lund Lund University  Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Eckermann","sequence":"additional","affiliation":[{"name":"ESRF, The European Synchrotron  Grenoble France"},{"name":"Institute for X\u2010Ray Physics, University of G\u00f6ttingen  G\u00f6ttingen Germany"},{"name":"Cluster of Excellence \u201cMultiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells\u201d (MBExC), University of G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasper","family":"Frohn","sequence":"additional","affiliation":[{"name":"Institute for X\u2010Ray Physics, University of G\u00f6ttingen  G\u00f6ttingen Germany"},{"name":"Cluster of Excellence \u201cMultiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells\u201d (MBExC), University of G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Salditt","sequence":"additional","affiliation":[{"name":"Institute for X\u2010Ray Physics, University of G\u00f6ttingen  G\u00f6ttingen Germany"},{"name":"Cluster of Excellence \u201cMultiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells\u201d (MBExC), University of G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bodil","family":"Ohlsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Malm\u00f6 Lund University  Lund Sweden"},{"name":"Department of Internal Medicine Sk\u00e5ne University Hospital  Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9109-7175","authenticated-orcid":false,"given":"Martin","family":"Bech","sequence":"additional","affiliation":[{"name":"Medical Radiation Physics, Department of Clinical Sciences Lund Lund University  Lund Sweden"},{"name":"LINXS Institute of advanced Neutron and X\u2010ray Science (LINXS)  Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0897-4_3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01230.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.200444"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2018.7"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14715"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12185968"},{"key":"e_1_2_8_8_1","first-page":"175","article-title":"Quantitative study of the development and maturation of human oesophageal innervation","volume":"180","author":"Hitchcock RJ","year":"1992","journal-title":"J. Anat."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.37059"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2012.706826"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2020.1815079"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v28.i29.3994"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15013"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801678115"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1600577520011327"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1600577520002398"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0582-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.10.036"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.148296"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113835118"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.02.035"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00432.2020"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1600577518010172"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000003293"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2022.837462"}],"container-title":["JGH Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgh3.70027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T10:00:45Z","timestamp":1727431245000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgh3.70027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/jgh3.70027"],"URL":"https:\/\/doi.org\/10.1002\/jgh3.70027","archive":["Portico"],"relation":{},"ISSN":["2397-9070","2397-9070"],"issn-type":[{"value":"2397-9070","type":"print"},{"value":"2397-9070","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2024-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70027"}}