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This review aims to highlight the limitations of traditional methods in diagnosing pancreatic disorders and cancer and explore several next\u2010generation sequencing (NGS) approaches as a promising alternative. There are distinct clinical symptoms that are shared by a number of clinical phenotypes of pancreatic illness induced by particular genetic mutations. Traditional diagnostic methods encompass computed tomography, magnetic resonance imaging, contrast\u2010enhanced Doppler ultrasound, endoscopic ultrasound, endoscopic retrograde cholangiopancreatography, transabdominal ultrasound, laparoscopy, and positron emission tomography have a prognostic ability of only 5% or less and a 5\u2010year survival rate. Genetic sequencing can be employed as an alternative to conventional diagnostic techniques. Sanger sequencing and NGS are currently largely operated genome analysis, with no exception for pancreatic disease diagnosis. The NGS methods can sequence millions to billions of short DNA fragments, enabling enormous sample screening in a short amount of time with low\u2010abundance detection, like in 0.1%\u20131% mutation prevalence declining approximate cost. Whole\u2010genome sequencing, whole\u2010exome sequencing, RNA sequencing, and single\u2010cell NGS are a few NGS methods utilized to diagnose pancreatic disease. For both research and clinical applications, the NGS techniques can provide a precise diagnosis of pancreatic disorders in a short amount of time at a reasonable expenditure.<\/jats:p>","DOI":"10.1002\/jgh3.70061","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T09:43:18Z","timestamp":1732700598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Next\u2010Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease\/Disorder"],"prefix":"10.1002","volume":"8","author":[{"given":"Suvro","family":"Biswas","sequence":"first","affiliation":[{"name":"Miocrobiology Laboratory, Department of Genetic Engineering and Biotechnology University of Rajshahi  Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamima","family":"Afrose","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohasana Akter","family":"Mita","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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