{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:37:31Z","timestamp":1787773051895,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014899","name":"American Neurogastroenterology and Motility Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014899","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003522","name":"Crohn's and Colitis UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003522","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020021","name":"Avant Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001779","name":"Monash University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001779","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1176553"],"award-info":[{"award-number":["APP1176553"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JGH Open"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The utility of circulating cytokine concentrations in ulcerative colitis is limited due to poor assay sensitivity. We aimed to examine the relationship of circulating cytokine concentrations, when measured by ultrasensitive detection, to disease activity, response to induction therapy, and physiological stimuli associated with mucosal injurious events.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma was obtained from adult patients with ulcerative colitis in whom disease activity was assessed; before\/after induction therapy; and before\/after acute psychological stress and cervical transcutaneous vagal nerve\/sham stimulation. Cytokines were measured using an ultrasensitive electrochemiluminescence\u2010based multiplex assay. For stress\/vagal\u2010stimulation studies, epithelial injury was quantified by plasma concentrations of intestinal\u2010type fatty acid\u2010binding protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 12 patients in remission, 15 with mild\u2010moderately active disease and 6 with severe colitis, concentrations of interleukin\u201017, IL10, interferon\u2010<jats:bold>\u03b3<\/jats:bold>, interleukin\u20108 and interleukin\u20106, but not tumor necrosis factor\u2010\u03b1 or interleukin\u201012p70, significantly reflected disease activity. In five patients in whom clinical remission was achieved with induction therapy, only interleukin\u201012p70 changed with a median increase of 65 (IQR 42\u2013303)% (<jats:italic>p<\/jats:italic>\u2009=\u20090.006), with no consistent changes in the 10 not in remission. Vagal nerve stimulation had no effect on cytokine concentrations, but, following stress, interleukin\u201012p70 increased by 37 (13\u201360)% compared with a reduction of 29 (3\u201355)% following sham stimulation (<jats:italic>p<\/jats:italic>\u2009=\u20090.012), an effect that mirrored that of epithelial injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Multiple cytokines were detected at sub\u2010pg\/mL levels and many showed relationships to disease activity in patients with ulcerative colitis. Effects exerted by interventions associated with epithelial injury were consistently detected by changes in interleukin\u201012p70 concentrations, but not other cytokines.<\/jats:p><jats:p><jats:bold>Trial Registration:<\/jats:bold> Australian New Zealand Clinical Trials Registry: ACTRN12621000168853 and ANZCTR 12620000569909 [Ultrasound studies]; <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link> identifier: NCT03908073<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgh3.70206","type":"journal-article","created":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T02:33:48Z","timestamp":1751164428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Stress, Vagal Nerve Stimulation and Disease Activity on Circulating Cytokines, Quantified by an Ultrasensitive Technique, in Ulcerative Colitis: A Pilot Study"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8954-2978","authenticated-orcid":false,"given":"Tamara","family":"Mogilevski","sequence":"first","affiliation":[{"name":"Department of Gastroenterology School of Translational Medicine, Monash University and Alfred Health  Melbourne Australia"},{"name":"The Royal London Hospital, Barts Health NHS Trust  London UK"},{"name":"Centre for Neuroscience, Surgery and Trauma Blizard Institute, Wingate Institute of Neurogastroenterology, Barts and the London School of Medicine and Dentistry, Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4923-0678","authenticated-orcid":false,"given":"Melinda Y.","family":"Hardy","sequence":"additional","affiliation":[{"name":"Immunology Division Walter and Eliza Hall Institute  Melbourne 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L.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology School of Translational Medicine, Monash University and Alfred Health  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1902-2640","authenticated-orcid":false,"given":"Adam","family":"Farmer","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology St Louis University  St Louis USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3353-9590","authenticated-orcid":false,"given":"James O.","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Centre for Neuroscience, Surgery and Trauma Blizard Institute, Wingate Institute of Neurogastroenterology, Barts and the London School of Medicine and Dentistry, Queen Mary University of London  London 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