{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:44:18Z","timestamp":1780526658589,"version":"3.54.1"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112\u20102314\u2010B\u2010182\u2010044"],"award-info":[{"award-number":["NSTC 112\u20102314\u2010B\u2010182\u2010044"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JGH Open"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Early detection is essential for improving hepatocellular carcinoma's (HCC) prognosis. However, the diagnostic performance of the current interventions remains unsatisfactory, particularly in patients with cirrhotic nodules. We aimed to develop a predictive model for the early detection of HCC using circulating tumor DNA (ctDNA) profiles based on 86 cancer\u2010related genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This model was initially evaluated on a discovery cohort of 25 patients with HCC and 9 patients with chronic hepatitis and then validated in a prospective control cohort of 15 alpha\u2010fetoprotein\u2010negative patients with cirrhotic nodules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the discovery cohort, our ctDNA\u2010based model achieved an area under the receiver operating characteristic curve of 0.8675, with a sensitivity of 88.0% and a specificity of 88.9%. CtDNA mutations in FGFR3, NF1, ALK, ERBB2, and NRAS were significantly associated with HCC prognosis. In the validation cohort, the ctDNA assay was positive in 8 of the 15 patients. Over a five\u2010year follow\u2010up period, 50% of ctDNA\u2010positive patients were diagnosed with early\u2010stage HCC, with a mean lead time of 26.6\u2009months compared to standard clinical assessments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>By enabling diagnosis months earlier than conventional methods, ctDNA\u2010based testing may enhance HCC surveillance and facilitate a more personalized approach to cancer monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgh3.70331","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T05:45:26Z","timestamp":1767073526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of Circulating Tumor\n                    <scp>DNA<\/scp>\n                    for Early Detection of Hepatocellular Carcinoma in Alpha\u2010Fetoprotein\u2013Negative Patients With Cirrhotic Nodules"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5962-627X","authenticated-orcid":false,"given":"Li\u2010Jun","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Master's Program in Clinical Trials and Assessment, College of Medicine Chang Gung University  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6980-7123","authenticated-orcid":false,"given":"Ming\u2010Chin","family":"Yu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medical Sciences Chang Gung University  Taoyuan Taiwan"},{"name":"College of Medicine Chang Gung University  Taoyuan Taiwan"},{"name":"Division of General Surgery Chang Gung Memorial Hospital, Linkou Medical Center  Taoyuan 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