{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:56:12Z","timestamp":1761918972473},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early clinical trials for gene therapy of human gliomas with retroviral packaging cells (PC) have been hampered by low transduction efficacy and lack of dissemination of PC within the tumor. In the current approach, these issues have been addressed by creating a stable packaging cell line for retroviral vectors pseudotyped with glycoproteins of lymphocytic choriomeningitis virus (LCMV) based on tumor\u2010infiltrating progenitor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tumor\u2010infiltrating progenitor cells, which had been isolated from adult rat bone marrow (BM\u2010TIC), were modified to stably express Gag\u2010Pol proteins of moloney murine leukemia virus (Mo\u2010MLV) and glycoproteins of LCMV. Packaging of a retroviral vector was measured by titration experiments on human fibroblast cells as well as on mouse and human glioma cell lines. Additionally, gene transfer was tested in a rat glioma model <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The BM\u2010TIC\u2010derived packaging cell line (BM\u2010TIPC) produced retroviral vectors with titers between 2\u20138 \u00d7 10<jats:sup>3<\/jats:sup> transducing units (TU)\/ml. Extended culturing of BM\u2010TIPC over several weeks and freezing\/thawing of cells did not affect vector titers. No replication\u2010competent retrovirus was released from BM\u2010TIPC. In a rat glioma model, BM\u2010TIPC infiltrated the tumors extensively and with high specificity. Moreover, BM\u2010TIPC mediated transduction of glioma cells <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This proof\u2010of\u2010principle study shows that primary adult progenitor cells with tumor\u2010infiltrating capacity can be genetically modified to stably produce retroviral LCMV pseudotype vectors. These BM\u2010TIPC may be a useful tool to enhance specificity and efficacy of gene transfer to gliomas in patients. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1032","type":"journal-article","created":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T13:19:04Z","timestamp":1178025544000},"page":"335-344","source":"Crossref","is-referenced-by-count":5,"title":["A retroviral packaging cell line for pseudotype vectors based on glioma\u2010infiltrating progenitor cells"],"prefix":"10.1002","volume":"9","author":[{"given":"Yvonne 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