{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:18:21Z","timestamp":1761862701138},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2007,9,17]],"date-time":"2007-09-17T00:00:00Z","timestamp":1189987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adiponectin (Adipo), an adipocyte hormone involved in the regulation of glucose and lipid metabolism, has already been identified as a potential therapeutic target for the treatment of diabetes. However, successful delivery of Adipo to the receptors is difficult due to their peptide characteristics. Receptors for Adipo are abundantly expressed in the liver and skeletal muscle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Uptake of 2\u2010(<jats:italic>N<\/jats:italic>\u2010(7\u2010nitrobenz\u20102\u2010oxa\u20101,3\u2010diazol\u20104\u2010yl)amino)\u20102\u2010deoxyglucose (2\u2010NBDG) in hepatoblastoma HepG2 cells expressing Adipo was examined. Adipo\u2010expressing plasmid DNA (10\u201350 \u00b5g) in saline solution (0.1 ml\/g body weight) was rapidly injected into the tail vein of 4\u2010week\u2010old diabetic mice after 4\u20136 weeks of treatment with streptozotocin (STZ). Uptake of glucose in diabetic mice also was measured using a planar positron imaging system featuring 18\u2010fluorodeoxyglucose.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HepG2 cells expressing Adipo exhibited significantly increased 2\u2010NBDG uptake compared with cells transfected with control plasmid even in the absence of insulin. STZ\u2010induced diabetic mice showed decreased serum Adipo levels compared with non\u2010diabetic mice. A single hydrodynamic injection of 10\u201350 \u00b5g Adipo\u2010expressing plasmid DNA into diabetic mice led to approximately 10\u201015\u2010fold elevation in serum Adipo levels, and resulted in decreased serum levels of glucose and triglyceride. As well as exhibiting higher levels of Adipo expression, diabetic mice also had higher hepatic glucose uptake than similar mice injected with control plasmid.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We report that STZ\u2010induced diabetic mice exhibited decreased Adipo levels and hyperglycemia which may be alleviated by hydrodynamic injection of the Adipo gene. This type of gene delivery system to the liver offers a different approach in developing novel treatments for type 1 and 2 diabetes. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1104","type":"journal-article","created":{"date-parts":[[2007,9,17]],"date-time":"2007-09-17T10:17:08Z","timestamp":1190024228000},"page":"976-985","source":"Crossref","is-referenced-by-count":30,"title":["Adiponectin gene therapy of streptozotocin\u2010induced diabetic mice using hydrodynamic injection"],"prefix":"10.1002","volume":"9","author":[{"given":"Masayoshi","family":"Fukushima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Tsukada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimiko","family":"Koga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichi","family":"Kajiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumi","family":"Kawano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneo","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuo","family":"Kamata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshie","family":"Maitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,9,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0587"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021483"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.18.10697"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.45.26746"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10842"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10583"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01705"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/90984"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2333912100"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.5.1304"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300947"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430349950017734"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.433"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i4.567"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.05.008"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm788"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700844"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15414.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02984486"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nima.2003.08.165"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0255"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.5.7463"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.26.12.3315"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.25.9.1665"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.27.8.2010"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/90992"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00118.2004"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00134.2005"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI14120"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402367200"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.FMJ04008X2"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.2005"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.821"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.277.1.E1"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00251-9"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.49.6.896"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.2005-0005"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302873"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.930"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjgm.1104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.1104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T15:26:15Z","timestamp":1700061975000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.1104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,17]]},"references-count":39,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1002\/jgm.1104"],"URL":"https:\/\/doi.org\/10.1002\/jgm.1104","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"value":"1099-498X","type":"print"},{"value":"1521-2254","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,17]]}}}