{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:34:18Z","timestamp":1781667258590,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,10,29]],"date-time":"2007-10-29T00:00:00Z","timestamp":1193616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We wanted to investigate the ability of recombinant equine infectious anemia virus (EIAV) vectors to transduce photoreceptor cells by developing a series of photoreceptor\u2010specific promoters that drive strong gene expression in photoreceptor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Promoter fragments derived from the rhodopsin (<jats:italic>RHO<\/jats:italic>), the beta phosphodiesterase (<jats:italic>PDE<\/jats:italic>) and the retinitis pigmentosa (<jats:italic>RP<\/jats:italic>1) genes were cloned in combination with an enhancer element, derived from the interphotoreceptor retinoid\u2010binding protein gene (<jats:italic>IRBP<\/jats:italic>), into luciferase reporter plasmids. An <jats:italic>in vitro<\/jats:italic> transient reporter assay was carried out in the human Y\u201079 retinoblastoma cell line. The optimal promoters from this screen were then cloned into the recombinant EIAV vector for evaluation <jats:italic>in vivo<\/jats:italic> following subretinal delivery into mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All promoters maintained a photoreceptor\u2010specific expression profile <jats:italic>in vitro<\/jats:italic> and the gene expression was further enhanced in combination with the IRBP enhancer. The use of IRBP\u2010combined <jats:italic>RHO<\/jats:italic> or <jats:italic>PDE<\/jats:italic> promoters showed modest but exclusive expression in photoreceptors following subretinal delivery to mice. By contrast an EIAV vector containing the cytomegalovirus (CMV) promoter drove reporter gene expression in both photoreceptors and retinal pigment epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>It may be possible to use recombinant EIAV vectors containing photoreceptor\u2010specific promoters to drive therapeutic gene expression to treat a range of retinal degenerative diseases where the photoreceptor cell is the primary disease target. 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