{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T19:49:53Z","timestamp":1774122593280,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T00:00:00Z","timestamp":1215561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Radiation\u2010induced, long\u2010lived free radicals, reactive oxygen species and pro\u2010inflammatory cytokines have been implicated in the resultant tissue injury after exposure to ionizing radiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An approach designed to reduce the damaging effects of reactive oxidants employs metalloenzymes of superoxide dismutase (SOD), such as MnSOD. Recombinant adeno\u2010associated virus 2 (AAV2) provides safe and long\u2010term expression in humans. We tested the effectiveness of AAV2\u2010MnSOD\u2010hrGFP, a vector expressing MnSOD and green fluorescent protein (GFP) in preclinical models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Infection of cultured cells with AAV2\u2010MnSOD\u2010hrGFP showed enhanced expression of MnSOD and GFP. Sustained expression of GFP was achieved for at least 1 month <jats:italic>in vivo<\/jats:italic> following administration of AAV2\u2010MnSOD\u2010hrGFP to subcutaneous tissue of C57BL\/6J mice. A single subcutaneous injection of AAV2\u2010MnSOD\u2010hrGFP significantly mitigated acute skin injury following single dose of irradiation of either 30 or 35 Gy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The proof\u2010of\u2010concept demonstrated in the present study together with the known safety profile in humans indicate that AAV\u2010mediated MnSOD expression has potential countermeasure utility against normal tissue injury following radiation therapy or radiological accident. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1226","type":"journal-article","created":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T08:05:47Z","timestamp":1215590747000},"page":"1012-1018","source":"Crossref","is-referenced-by-count":32,"title":["Mitigation of radiation\u2010induced skin injury by AAV2\u2010mediated MnSOD gene therapy"],"prefix":"10.1002","volume":"10","author":[{"given":"Shiqing","family":"Yan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Kolozsvary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svend O.","family":"Freytag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Ho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.07.001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2005.01.040"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-8140(94)90444-8"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2004.tb00265.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(03)01369-5"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-5-59"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300580"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2006.09.053"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.113.1_Supplement.46S"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/104454902762053855"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02982689"},{"key":"e_1_2_6_13_2","first-page":"363","article-title":"Gene therapy vectors based on adeno\u2010associated virus: characteristics and applications to acquired and inherited diseases (review)","volume":"6","author":"Athanasopoulos T","year":"2000","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)63504-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1667\/RR0707.1"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1081\/STA-100104756"},{"key":"e_1_2_6_17_2","first-page":"3803","article-title":"Prevention of radiation induced central nervous system toxicity: a role for amifostine?","volume":"24","author":"Nieder C","year":"2004","journal-title":"Anticancer Res"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-5115"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-10-3867"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2006.05.004"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302468"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.04.029"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/rpd\/ncl458"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199902000-00017"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.125.2.509"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303402760128577"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60982-9"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302011"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.11.057"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.12.9795-9805.1998"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302815"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.8.3852-3858.2000"},{"key":"e_1_2_6_33_2","first-page":"201","article-title":"AAV\u2010mediated gene transfer to mouse lungs","volume":"246","author":"Halbert CL","year":"2004","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02220.x"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2006.03.009"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.02.026"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2005.10.012"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1269\/jrr.46.305"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-4446"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjgm.1226","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.1226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T14:37:02Z","timestamp":1697639822000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.1226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,19]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1002\/jgm.1226"],"URL":"https:\/\/doi.org\/10.1002\/jgm.1226","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"value":"1099-498X","type":"print"},{"value":"1521-2254","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,19]]}}}