{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:07:15Z","timestamp":1773277635431,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eosinophilia plays the major role in the pathogenesis of asthma and correlates with the up\u2010regulation of eotaxin, which, together with interleukin (IL)\u20105, is important for differentiation, chemo\u2010attraction, degranulation, and survival of eosinophils in local tissue. In a previous study, we found that administration of lentivirus\u2010delivered short hairpin RNA (shRNA) to suppress the expression of IL\u20105 inhibited airway inflammation. The present study aimed to investigate the role of eotaxin shRNA and the synergistic effect of eotaxin and IL\u20105 shRNAs on airway inflammation in an ovalbumin (OVA)\u2010induced murine model of asthma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lentivirus\u2010delivered shRNAs were used to suppress the expression of eotaxin and\/or IL\u20105 in local tissue in an OVA\u2010induced murine asthma model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intra\u2010tracheal administration of lentivirus containing eotaxin shRNA expressing cassette (eoSEC3.3) efficiently moderated the characteristics of asthma, including airway hyper\u2010responsiveness, cellular infiltration of lung tissues, and eotaxin and IL\u20105 levels in bronchio\u2010alveolar lavage fluid. Administration of lentiviruses expressing IL\u20105 or eotaxin shRNAs (IL5SEC4 + eoSEC3.3) also moderated the symptoms of asthma in a mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Local delivery of lentiviruses expressing IL\u20105 and eotaxin shRNAs provides a potential tool in moderating airway inflammation and also has the potential for developing clinical therapy based on the application of shRNAs of chemokines and cytokines involved in T helper 2 cell inflammation and eosinophilia. 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