{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:48:31Z","timestamp":1761630511391},"reference-count":70,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,13]],"date-time":"2009-02-13T00:00:00Z","timestamp":1234483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Developing vectors that target specifically to disease sites after systemic injection is an important goal in gene therapy research.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prepared fluorescent DNA polyplexes (\u2a7d150 nm in diameter) comprising plasmid DNA condensed with poly(<jats:sc>L<\/jats:sc>\u2010lysine) and coated with a multivalent reactive copolymer based on poly[<jats:italic>N<\/jats:italic>\u2010(2\u2010hydroxypropyl)methacrylamide] (pHPMA). These polyplexes were then surface modified with a recombinant P\u2010selectin glycoprotein ligand\u20101 immunoglobulin chimera (rPSGL\u2010Ig) previously investigated as a selectin antagonist in clinical studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five minutes after jugular vein injection of these polyplexes, fluorescence accumulation in inflamed cremasteric venules of C57BL6 mice was more than eight\u2010fold higher than that observed after injection of Fc\u2010blocked control polyplexes. Fluorescence above background was not observed in P\u2010selectin deficient mice, confirming the specificity for P\u2010selectin in this model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data provide encouragement for the further development of rPSGL\u2010Ig\u2010coated polyplexes as potential nonviral vectors for targeted gene therapy in inflammatory conditions, such as ischaemia reperfusion injury, unstable atherosclerotic plaques and myocarditis. This approach may also be transferable to the use of other targeting ligands whose cognate partner is specifically upregulated on the vascular endothelium in individual pathological situations. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1294","type":"journal-article","created":{"date-parts":[[2009,2,13]],"date-time":"2009-02-13T11:57:41Z","timestamp":1234526261000},"page":"326-334","source":"Crossref","is-referenced-by-count":6,"title":["P\u2010selectin dependent targeting to inflamed endothelium of recombinant P\u2010selectin glycoprotein ligand\u20101 immunoglobulin chimera\u2010coated poly[<i>N<\/i>\u2010(2\u2010hydroxypropyl) methacrylamide]\u2010DNA polyplexes <i>in vivo<\/i> visualised by intravital microscopy"],"prefix":"10.1002","volume":"11","author":[{"given":"Christopher M.","family":"Newman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Crosdale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry 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