{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:11:19Z","timestamp":1782731479427,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T00:00:00Z","timestamp":1241654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Salivary glands have been proposed as target organs for gene therapy. They secrete endogenous, as well as transgenic proteins, in a polarized manner. Transgene\u2010encoded regulated pathway proteins primarily follow the regulated pathway in rat salivary glands and are secreted into saliva in an exocrine manner. Conversely, constitutive pathway proteins generally are secreted more basolaterally and thus follow the endocrine route. In the present study, we studied <jats:italic>in vivo<\/jats:italic> the sorting of the mouse immunoglobulin G2b Fc fragment, which is physiologically secreted via the constitutive pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adenoviral vectors encoding the Fc fragment and human growth hormone were delivered into rat and mouse submandibular glands <jats:italic>in vivo<\/jats:italic> to compare their serum\u2010to\u2010saliva distribution. We also compared the intracellular localization of the Fc fragment and growth hormone by confocal microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the Fc fragment was secreted almost entirely into the bloodstream from rat and mouse submandibular glands via a constitutive or constitutive\u2010like pathway. This sorting behaviour is clearly different from that of transgenic human growth hormone, which is secreted in a regulated pathway, both in neuroendocrine cells and as a transgenic protein from salivary gland cells. We also found that simultaneously expressed human growth hormone and the mouse Fc fragment do not appear to influence each other's sorting behaviour. The Fc fragment showed a primarily basal localization, whereas growth hormone showed an apical localization, in rat submandibular gland acinar cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results obtained in the present study indicate that the mouse Fc fragment is a useful model protein for examining the basolateral versus apical secretory pathways employed by transgenic secretory proteins in salivary glands. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1340","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T09:10:58Z","timestamp":1241687458000},"page":"580-587","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vivo<\/i> secretion of the mouse immunoglobulin G Fc fragment from rat submandibular glands"],"prefix":"10.1002","volume":"11","author":[{"given":"Gabor Z.","family":"Racz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Perez\u2010Riveros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janik","family":"Adriaansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Baum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,5,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430349950016528"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.11.002"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.18-2255"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.17-2177"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400604"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090010301"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1242\/jcs.109.10.2591","article-title":"Two regulated secretory pathways for newly synthesized parotid salivary proteins are distinguished by doses of secretagogues","volume":"109","author":"Castle JD","year":"1996","journal-title":"J Cell Sci"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3320593"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2007.0136"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06171"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400136101"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2008.034"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.05.177"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.56"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01398-2"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.2.239"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300622"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.22070"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303401750270986"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.571"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.276.2.E223"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.12.4.7695917"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02737888"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2994224"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.1.3"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.17"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjgm.1340","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.1340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T07:40:19Z","timestamp":1696923619000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.1340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,7]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1002\/jgm.1340"],"URL":"https:\/\/doi.org\/10.1002\/jgm.1340","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"value":"1099-498X","type":"print"},{"value":"1521-2254","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,7]]}}}