{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:22:24Z","timestamp":1785198144750,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T00:00:00Z","timestamp":1265328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dendritic cells (DCs) constitute an attractive target for immunotherapeutic approaches. Because DCs are largely refractory to transfection with plasmid DNA, several viral transduction protocols were established. The potential side\u2010effects of lentiviral transduction on the phenotype and activation state of DCs left unstimulated after transduction have not been assessed. There is a need to analyse these parameters as a result of the requirement of using DCs with a low activation state for therapeutic strategies intended to induce tolerance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lentivirally\u2010transduced bone marrow (BM)\u2010derived DCs (LV\u2010DCs) in comparison with mock\u2010transduced (Mock\u2010DCs) and untreated DCs were analysed with regard to the induction of maturation processes on the RNA, protein and functional level. BM\u2010DCs engineered to overexpress interleukin (IL)\u201010 were analysed for therapeutic potential in a mouse model of allergic contact dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with untreated DCs, Mock\u2010DCs and LV\u2010DCs displayed an altered gene expression signature. Mock\u2010DCs induced a stronger T cell proliferative response than untreated DCs. LV\u2010DCs did not further augment the T cell proliferative response, but induced a slightly different T cell cytokine pattern compared to Mock\u2010DCs. Accordingly, the gene promoter of the DC maturation marker fascin mediated efficient expression of the model transgene IL\u201010 in unstimulated\u2010transduced BM\u2010DCs. Nevertheless, IL\u201010 overexpressing BM\u2010DCs exerted tolerogenic activity and efficiently inhibited the contact hypersensitivity response in previously hapten\u2010sensitized mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Lentiviral transduction of BM\u2010DCs results in their partial activation. Nevertheless, the transduction of these DCs with a vector encoding the immunomodulatory cytokine IL\u201010 rendered them tolerogenic. Thus, lentivirally\u2010transduced DCs expressing immunomodulatory molecules represent a promising tool for induction of tolerance. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1436","type":"journal-article","created":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T09:58:38Z","timestamp":1265363918000},"page":"231-243","source":"Crossref","is-referenced-by-count":16,"title":["Dendritic cells lentivirally engineered to overexpress interleukin\u201010 inhibit contact hypersensitivity responses, despite their partial activation induced by transduction\u2010associated physical 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