{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:12:24Z","timestamp":1791220344327,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T00:00:00Z","timestamp":1271116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adenoviruses can cause severe toxicity in children and in immunocompromised adults, and therefore a means to abrogate replication would be useful. With regard to cancer treatment, replication competent oncolytic adenoviruses have been safe in humans, although their efficacy has been variable. Therefore, more effective agents are now entering clinical testing and, consequently, replication\u2010associated side effects remain a concern. Preclinical analysis of replication related toxicity has been hampered by a lack of permissive models. Therefore, it has been difficult to study modulation of human adenovirus replication in immune competent animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated four different hamster carcinoma cell lines for transduction and cell killing potency <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Gene transfer was assessed using replication\u2010deficient adenoviruses expressing luciferase. Cell killing was studied <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using an oncolytic adenovirus that kills tumor cells by viral replication. After the most promising animal model had been selected, abrogation of virus replication was assessed <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using a TCID<jats:sub>50<\/jats:sub> assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results obtained suggest wild\u2010type adenovirus replication in all four tested Syrian hamster cell lines and also normal organs. Virus replication could be abrogated with chlorpromazine, cidofovir and cytosine arabinoside, and the effect occurred subsequent to nuclear delivery of the viral genome. Attenuation of virus replication also was seen <jats:italic>in vivo<\/jats:italic> both in tumors and the liver.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Syrian hamsters may comprise a valuable immune competent model for evaluating anti\u2010adenoviral drugs. Furthermore, chlorpromazine or cidofovir might be useful in case of adenovirus replication\u2010associated symptoms in humans. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1453","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T13:58:40Z","timestamp":1271167120000},"page":"435-445","source":"Crossref","is-referenced-by-count":36,"title":["Human adenovirus replication in immunocompetent Syrian hamsters can be attenuated with chlorpromazine or cidofovir"],"prefix":"10.1002","volume":"12","author":[{"given":"Iulia","family":"Diaconu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Cerullo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Escutenaire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kanerva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd 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