{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:54:02Z","timestamp":1783486442928,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,1,24]],"date-time":"2011-01-24T00:00:00Z","timestamp":1295827200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Autoimmune thyroiditis is one of common organ\u2010specific autoimmune disease. The aim of this study was to observe the effect of adipose tissue derived mesenchymal stem cells (ATMSC) and CTLA4Ig gene\u2010transduced ATMSC on autoimmune thyroiditis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Experimental autoimmune thyroiditis was induced by immunization with thyroglobulin. Animals were divided into three groups: (i) a half million of human ATMSC, (ii) a half million of murine CLTA4Ig gene\u2010transduced human ATMSC (CTLA4Ig\u2010MSC), or (iii) normal saline (as control), which were administered intravenously four times within a 3\u2010week period. Blood and tissue samples were collected 1 week after the last cell transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The absorbance of serum thyroglobulin autoantibody (TgAA) in the CTLA4Ig\u2010MSC group was considerably lower than those in other groups. In culture supernatant of LPS\u2010stimulated spleen cells, both of the MSC\u2010treated groups showed significantly lower absorbances of TgAA than the control. Flow cytometric analysis of spleen cells revealed a significant decrease in the proportion of CD3+ and CD11b in the CTLA4Ig\u2010MSC group compared to the other groups. Lymphocyte infiltration in the thyroid glands was also dramatically decreased in both of MSC\u2010treated groups. Cytokine analysis showed that ATMSC decreased the production of proinflammatory cytokines and improved the Th1\/Th2 balance by down\u2010regulating Th1 cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although CTLA4Ig\u2010MSC transplantation had better result in reduction of serum TgAA, both of ATMSC and CTLA4Ig\u2010MSC transplantations are promising treatments for autoimmune thyroiditis judging from the results of histopathology and cytokine analysis. They may be attractive candidates for treating organ\u2010specific autoimmune disease. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.1531","type":"journal-article","created":{"date-parts":[[2010,11,26]],"date-time":"2010-11-26T02:49:20Z","timestamp":1290739760000},"page":"3-16","source":"Crossref","is-referenced-by-count":48,"title":["Transplantation of CTLA4Ig gene\u2010transduced adipose tissue\u2010derived mesenchymal stem cells reduces inflammatory immune response and improves Th1\/Th2 balance in experimental autoimmune thyroiditis"],"prefix":"10.1002","volume":"13","author":[{"given":"Eun Wha","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il Seob","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Woo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So Young","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi Hyun","family":"Nam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Sung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Kyu","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Ji","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Keun","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Chan","family":"Ra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwa Young","family":"Youn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hwa","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,1,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1997.4412"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-9972(03)00006-5"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.4.1681"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03187.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.10.3838"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2657"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0359"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0586"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1272\/jnms.76.56"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2007.0153"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2009.2.2.122"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2004.06.002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2008.34256.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10350-005-0052-6"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/SAP.0b013e31817f01b6"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0228"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24405"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.168534"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1496399"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1323143"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1801"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.12.3815.3815"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7520604"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1203"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/14653240902960429"},{"issue":"12","key":"e_1_2_6_28_2","doi-asserted-by":"crossref","first-page":"6634","DOI":"10.4049\/jimmunol.154.12.6634","article-title":"Curative and protective effects of IL\u201010 in experimental autoimmune thyroiditis (EAT). 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