{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:04:20Z","timestamp":1792623860008,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T00:00:00Z","timestamp":1343174400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The current treatment of ulcerative colitis (UC) is less than ideal and has room for improvement. Bone morphogenetic protein\u20107 (BMP\u20107) exerts a protective effect on experimental UC. Hence, we considered that intracolonically (i.c.) administered adeno\u2010associated virus (AAV) delivering BMP\u20107 might have therapeutic potential for UC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Recombinant AAV type 2 vectors carrying enhanced green fluorescence protein (AAV\u2010EGFP), LacZ (AAV\u2010LacZ) and BMP\u20107 (AAV\u2010BMP\u20107) were generated. Bioluminescence imaging, \u03b2\u2010galactosidase assay and western blotting were applied to determine the colonic expression of EGFP, LacZ and BMP\u20107, respectively, after i.c. administration of the AAVs. Disease activity index (DAI) was observed daily during the 7\u2009days of dextran sulphate sodium (DSS) treatment initiated 4\u2009days after i.c. AAV\u2010BMP\u20107, AAV\u2010LacZ or phosphate\u2010buffered saline. The colonic pathological morphology, mucosal myeloperoxidase (MPO) activity, malondialdehyde content, superoxide dismutase activity and proliferating cell nuclear antigen were determined at the end of DSS treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When i.c administered to rats, AAV could efficiently transduce the colonic mucosa. Enema with AAV\u2010BMP\u20107 significantly ameliorated DSS\u2010induced colitis as indicated by reduced DAI, decreased macroscopic and histological scores and declined MPO activity compared to the controls. Furthermore i.c. AAV\u2010BMP\u20107 significantly prevented oxidant damage and attenuated complementary mucosal cell proliferation in the DSS\u2010treated rat colons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data demonstrate that i.c. administration of AAV\u2010BMP\u20107 efficiently mediates the ectopic BMP\u20107 expression in rat colon and further ameliorates DSS\u2010induced UC in rats, suggesting that i.c. AAV\u2010BMP\u20107 has the potential to be developed into an alternative therapeutic measure for the treatment of UC. 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