{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:52:24Z","timestamp":1770511944243,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,8,25]],"date-time":"2012-08-25T00:00:00Z","timestamp":1345852800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Our previous study showed an efficient targeting of islets of Langerhans by adenoviral injection via the celiac trunk. Unexpectedly, none of the endothelial cells was infected given the direct contact between adenoviruses and the capillary wall. The present study intended to provide an efficient approach for adenoviral targeting of the microcapillary endothelial cells in the pancreas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prepared microspheres of poly(3\u2010hydroxybutyrate\u2010co\u20103\u2010hydroxyhexanoate) (PHBHHx) with a size comparable to the diameter of capillary (5\u201310\u2009\u00b5m). Scanning electron microscopy was applied to verify that adenoviruses carrying a green fluorescence protein gene were complexed with PHBHHx\u2010microspheres after 30\u2009min of co\u2010incubation. The complexes were then injected into the pancreas of mice via the celiac trunk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Approximately 40% of endothelial cells in the pancreas were labeled 5\u2009days after surgery. Islet cells were labeled occasionally, whereas labeling of the acinar and ductal tissues was barely detectable. Endothelium targeting was inefficient in other internal organs. Consistent with the reported superior tissue compatibility of PHBHHx, no discernable microspheres were found in all of the organs examined. Furthermore, splenocyte activation was dampened when adenoviruses were complexed with the microspheres.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study has established an approach for efficient pancreatic capillary targeting by using microsphere\u2010adenoviral complexes. This procedure could be invaluable for the treatment of capillary\u2010related diseases. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2650","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T03:08:32Z","timestamp":1342667312000},"page":"530-539","source":"Crossref","is-referenced-by-count":8,"title":["Transient embolization with microspheres of polyhydroxyalkanoate renders efficient adenoviral transduction of pancreatic capillary<i>in vivo<\/i>"],"prefix":"10.1002","volume":"14","author":[{"given":"Shui\u2010Li","family":"Zhang","sequence":"first","affiliation":[{"name":"Multidisciplinary Research Center Shantou University Shantou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u2010Jin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Multidisciplinary Research Center Shantou University Shantou Guangdong 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