{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T21:52:21Z","timestamp":1777153941086,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,4,24]],"date-time":"2002-04-24T00:00:00Z","timestamp":1019606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Transduction of the murine retinal pigmented epithelium (RPE) with adenovirus vectors requires technically difficult and invasive subretinal injections. This study tested the hypothesis that recombinant vectors based on feline immunodeficiency virus (FIV) could access the retina following intravitreal injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>FIV vectors expressing <jats:italic>E. coli<\/jats:italic> \u03b2\u2010galactosidase (FIV\u03b2gal) were injected alone, or in combination with adenovirus vectors expressing eGFP, into the vitreous of normal mice and eyes evaluated for transgene expression. In further studies, the utility of FIV\u2010mediated gene transfer to correct lysosomal storage defects in the anterior and posterior chambers of eyes was tested using recombinant FIV vectors expressing \u03b2\u2010glucuronidase. FIV\u03b2gluc vectors were injected into \u03b2\u2010glucuronidase\u2010deficient mice, an animal model of mucopolysacharridoses type VII.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results of this study show that similar to adenovirus, both corneal endothelium and cells of the iris could be transduced following intravitreal injection of FIV\u03b2gal. However, in contrast to adenovirus, intravitreal injection of FIV\u03b2gal also resulted in transduction of the RPE. Immunohistochemistry following an intravitreal injection of an AdeGFP (adenovirus expressing green fluorescent protein) and FIV\u03b2gal mixture confirmed that both viruses mediated transduction of corneal endothelium and cells of the iris, while only FIV\u03b2gal transduced cells in the retina. Using the \u03b2\u2010glucuronidase\u2010deficient mouse, the therapeutic efficacy of intravitreal injection of FIV\u03b2gluc (FIV expressing \u03b2\u2010glucuronidase) was tested. Intravitreal injection of FIV\u03b2gluc to the eyes of \u03b2\u2010glucuronidase\u2010deficient mice resulted in rapid reduction (within 2\u2009weeks) of the lysosomal storage defect within the RPE, corneal endothelium, and the non\u2010pigmented epithelium of the ciliary process. Transgene expression and correction of the lysosomal storage defect remained for at least 12\u2009weeks, the latest time point tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These studies demonstrate that intravitreal injection of FIV\u2010based vectors can mediate efficient and lasting transduction of cells in the cornea, iris, and retina. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.267","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T22:46:39Z","timestamp":1034117199000},"page":"463-469","source":"Crossref","is-referenced-by-count":23,"title":["Feline immunodeficiency virus vectors. 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