{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:02:08Z","timestamp":1773277328569,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T00:00:00Z","timestamp":1393286400000},"content-version":"vor","delay-in-days":55,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recombinant adeno\u2010associated virus (rAAV) is recognized as a promising vector for cancer gene therapy, although its low transfer efficiency in less permissive cells limits extensive application. Our previous studies reported that ultrasound\u2010targeted microbubble (MB) destruction (UTMD) enhanced rAAV transfer in its permissive retinal cells. In the present study, we investigated whether UTMD increased rAAV transfer in less permissive human renal cell carcinoma (hRCC) cells and tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>hRCC cells were treated with rAAV2 under different conditions of UTMD, and the viral transfer efficiency and cell viability were analyzed. Fifty\u2010two male nude mice (BALB\/c) implanted with hRCC cells were randomly assigned to four groups consisting of rAAV, rAAV\u2009+\u2009ultrasound and rAAV\u2009+\u2009UTMD (20\u2009\u00b5l and 40\u2009\u00b5l of MBs). UTMD was initiated immediately after intratumoral viral injection, and viral transfer efficiency and tumor volumes were analyzed at 12\u2009weeks after infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The efficiency of non\u2010augmented transfer of rAAV2 into hRCC cells was low (17.28\u2009\u00b1\u20092.44%). The use of UTMD enhanced viral transfer efficiency by two\u2010 to three\u2010fold, and enhanced viral genomic DNA by more than nine\u2010fold, without decreasing cell viability. <jats:italic>In vivo<\/jats:italic> studies also showed that UTMD increased rAAV2 transfer in tumor. The enhancements were maintained for a period of 12\u2009weeks. Tumor growth in mice was inhibited by UTMD treatment, and UTMD treatment augmented by MBs (40\u2009\u00b5l) produced an even stronger effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>UTMD enhanced rAAV2 transfer into less permissive RCC cells and tumors, resulting in inhibition of tumor growth, which suggests that UTMD may be a useful delivery tool for cancer gene therapy. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2755","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T06:36:55Z","timestamp":1390804615000},"page":"28-39","source":"Crossref","is-referenced-by-count":16,"title":["The dual effect of ultrasound\u2010targeted microbubble destruction in mediating recombinant adeno\u2010associated virus delivery in renal cell carcinoma: transfection enhancement and tumor inhibition"],"prefix":"10.1002","volume":"16","author":[{"given":"Fan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Ultrasound Shanghai Jiaotong University Affiliated First People's Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Shanghai Jiaotong University Affiliated First People's Hospital  Shanghai 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