{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T14:21:18Z","timestamp":1751466078877},"reference-count":35,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T00:00:00Z","timestamp":1442448000000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2015,8,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adoptive transfer of CD8<jats:sup>+<\/jats:sup> T\u2010cells specific for tumor\u2010antigens is an attractive strategy for anti\u2010tumor therapy. In the present study, the subsets T<jats:sub>A<\/jats:sub> and T<jats:sub>B<\/jats:sub> were used to represent the population of CD8<jats:sup>+<\/jats:sup> T cells generated by culturing the respective cells with irradiated dendritic cells (DCs) pulsed with ovalbumin (OVA) protein and transfected with adenoviral vector constructs as described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Na\u00efve OVA specific CD8<jats:sup>+<\/jats:sup> T cells were isolated from the spleen of OVA\u2010specific T\u2010cell receptor transgenic OTI mice. The subsets T<jats:sub>A<\/jats:sub> and T<jats:sub>B<\/jats:sub> were then generated by <jats:italic>in vitro<\/jats:italic> activating the population of CD8<jats:sup>+<\/jats:sup> T cells with OVA\u2010pulsed DCs transfected with <jats:italic>IL\u20106<\/jats:italic>\u2010expressing adenoviral vector (AdV<jats:italic><jats:sub>IL\u20106<\/jats:sub><\/jats:italic>) or the control vector (AdV<jats:sub>Null<\/jats:sub>). To assess their <jats:italic>in vivo<\/jats:italic> immunotherapeutic effects, T<jats:sub>A<\/jats:sub>\u2010 or T<jats:sub>B<\/jats:sub>\u2010cells were intravenously transferred into C57BL\/6 mice bearing EG7 thymoma (6\u20138\u2009mm in diameter).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>T<jats:sub>A<\/jats:sub>\u2010cells displayed a higher level of expression of CD62\u2009l, IL\u20107R, FasL, perforin and CCR6, and also exhibited more potent <jats:italic>in vitro<\/jats:italic> cytotoxicity to OVA\u2010expressing EG7 thymoma cells via perforin\u2010 and Fas\/FasL\u2010mediated apoptosis than T<jats:sub>B<\/jats:sub>\u2010cells. CD8<jats:sup>+<\/jats:sup> T\u2010cell survival was kinetically analyzed in C57BL\/6 mice transferred with T<jats:sub>A<\/jats:sub>\u2010 or T<jats:sub>B<\/jats:sub>\u2010cells by flow cytometry. We found that the adoptively transferred T<jats:sub>A<\/jats:sub>\u2010cells had prolonged survival and enhanced T\u2010cell memory development compared to T<jats:sub>B<\/jats:sub>\u2010cells. In addition, T<jats:sub>A<\/jats:sub>\u2010, but not T<jats:sub>B<\/jats:sub>\u2010cells were able to eradicate well\u2010established EG7 thymomas in all eight tumor\u2010bearing mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data suggest that AdV<jats:italic><jats:sub>IL\u20106<\/jats:sub><\/jats:italic>\u2010transfected DC\u2010stimulated CD8<jats:sup>+<\/jats:sup> T cells with potent cytotoxicity and survival advantage may serve as an effective adoptive CD8<jats:sup>+<\/jats:sup> T\u2010cell immunotherapy strategy for anti\u2010tumor treatment. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2836","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T16:21:33Z","timestamp":1437754893000},"page":"153-160","source":"Crossref","is-referenced-by-count":6,"title":["Potent immunotherapy against well\u2010established thymoma using adoptively transferred transgene <i>IL\u20106<\/i>\u2010engineered dendritic cell\u2010stimulated CD8<sup>+<\/sup> T\u2010cells with prolonged survival and enhanced cytotoxicity"],"prefix":"10.1002","volume":"17","author":[{"given":"Kalpana","family":"Kalyanasundaram Bhanumathy","sequence":"first","affiliation":[{"name":"Cancer Research Cluster, Saskatchewan Cancer Agency, Division of Oncology University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Research Cluster, Saskatchewan Cancer 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