{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:27:59Z","timestamp":1780396079431,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"4-6","license":[{"start":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T00:00:00Z","timestamp":1465171200000},"content-version":"vor","delay-in-days":66,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A successful clinical outcome for implanted tissue\u2010engineered bone is dependent on the establishment of a functional vascular network. Gene\u2010enhanced tissue engineering represents a promising approach for vascularization and osteogenesis. In the present study, we tested the angiogenesis and osteogenesis efficacy of gelatin as the scaffold carrier in combination with a virus encoding the HIF\u20101\u03b1 gene in a rat alveolar bone defect model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three groups of 10 rats each were either left untreated, treated with adenovirus encoding hypoxia\u2010inducible factor\u20101\u03b1 (AdHIF\u20101\u03b1)\/gelatin sponge or treated with gelatin sponge with adenovirus encoding red fluorescence protein, respectively. At 4 weeks, all samples were determined by micro\u2010computed tomography, histological analyses and immunohistochemical studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Scaffolds loaded with AdHIF\u20101\u03b1 were able to sustain the release of AdHIF\u20101\u03b1 for up to 21 days and alveolar bone defects treated with scaffolds containing AdHIF\u20101\u03b1 significantly induced new bone and new vessel formation <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overexpression of HIF\u20101\u03b1 by gene therapy may be a useful method for enhancing alveolar bone defect osteogenesis and angiogenesis. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2876","type":"journal-article","created":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:42:49Z","timestamp":1456792969000},"page":"57-64","source":"Crossref","is-referenced-by-count":20,"title":["Application of HIF\u20101\u03b1 by gene therapy enhances angiogenesis and osteogenesis in alveolar bone defect regeneration"],"prefix":"10.1002","volume":"18","author":[{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Huang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhong","family":"Hu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyue","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Xu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"},{"name":"Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12395"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2015.01.006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2015.02.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm401825c"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200900107"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0138"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2015.00004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-009-0477-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/6\/1\/015013"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.33009"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.06.029"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1109\/MEMB.2003.1256276"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.005"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.202"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2010.0453"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.11607\/prd.0868"},{"key":"e_1_2_6_18_1","first-page":"269","article-title":"Expression of various growth factors for cell proliferation and cytodifferentiation during fracture repair of bone","volume":"44","author":"Tatsuyama K","year":"2000","journal-title":"Eur J Histochem"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911921106"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/JIM.0000000000000206"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032355"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2015.09.021"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2013.08.015"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.10.029"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arthro.2015.03.008"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2012.10.001"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2015.00009"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.03.013"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623307309925"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.02.018"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjgm.2876","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.2876","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T17:23:59Z","timestamp":1695749039000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.2876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":29,"journal-issue":{"issue":"4-6","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1002\/jgm.2876"],"URL":"https:\/\/doi.org\/10.1002\/jgm.2876","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"value":"1099-498X","type":"print"},{"value":"1521-2254","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4]]}}}