{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T02:41:17Z","timestamp":1773456077151,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T00:00:00Z","timestamp":1467676800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National High Technology Program","award":["2012AA020801"],"award-info":[{"award-number":["2012AA020801"]}]},{"name":"National twelfth \u2018five years\u2019 project in Science and Technology","award":["2013ZX10002-003"],"award-info":[{"award-number":["2013ZX10002-003"]}]},{"name":"Innovation Team Development Plan of the Ministry of Education of China","award":["IRT1131"],"award-info":[{"award-number":["IRT1131"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Human fibrinogen\u2010like protein 2 (hFGL2) is overexpressed in various human solid tumors and is associated with tumor growth, invasion and angiogenesis of hepatocellular carcinoma (HCC) LM6 cells. In the present study, an adenoviral vector expressing an artificial microRNA (miRNA) against hfgl2 (Ad\u2010hfgl2\u2010miRNA) was used to evaluate the effect of FGL2 knockdown on cell proliferation and tumor growth of HCCLM6 xenografts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ad\u2010hfgl2\u2010miRNA was constructed and its effect on hFGL2 expression and cell proliferation was analyzed. The xenograft tumor model was established in nude mice and this was accompanied by intratumoral injection of Ad\u2010hfgl2\u2010miRNA when the tumor volume had reached 100\u2009mm<jats:sup>3<\/jats:sup>. The volume of the tumor xenograft was recorded and angiogenesis in the tumor xenograft was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ad\u2010hfgl2\u2010miRNA significantly downregulated hFGL2 expression at both the mRNA and protein levels and exerted an inhibitory effect on proliferation in HCCLM6 cells. In HCCLM6 tumor\u2010bearing mice, the tumor volume significantly decreased following the intratumoral injection of Ad\u2010hfgl2\u2010miRNA compared to that of control groups. Additionally, Ad\u2010hfgl2\u2010miRNA also exhibited potent anti\u2010angiogenic effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HFGL2 might be a potential target for controlling angiogenesis in HCC and hfgl2 gene silencing via miRNA may be an alternative strategy for the management of HCC. 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