{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:00:53Z","timestamp":1776258053192,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T00:00:00Z","timestamp":1471478400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Delivery of bone morphogenetic protein\u20107 (BMP\u20107) to bone defects can be improved by applying gene transfer methods. However, traditional <jats:italic>ex vivo<\/jats:italic> gene therapy approaches are cumbersome and costly, requiring the extraction and culturing of cells. Therefore, we evaluated a novel, expedited <jats:italic>ex vivo<\/jats:italic> BMP\u20107 gene transfer technology based on the use of fragments of subcutaneous fat tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We created 5\u2010mm mid\u2010femoral bone defects in the right femora of 23 male, syngeneic Fischer 344 rats. Adipose tissue was harvested from the subcutaneous fat depot of two donor rats. Bone defects were treated with either unmodified fat (control group) or adenovirally BMP\u20107 transduced fat fragments (treatment group). Healing of bone defects was assessed by radiographs, microcomputed tomography (\u03bcCT) and histology at 6\u2009weeks after the implantation of fat tissue fragments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Radiographs, \u03bcCT\u2010imaging and histology revealed relevant bone formation in six out of 10 rats treated with BMP\u20107 activated fat grafts. Two of the defects were bridged. By contrast, femora of the control group receiving unmodified fat did not display signs of osseous healing. BMP\u20107 gene activated fat treatment led to a significantly higher bone volume (11.18\u2009\u00b1\u20099.48\u2009mm<jats:sup>3<\/jats:sup>) than treatment with unmodified fat grafts (3.19\u2009\u00b1\u20091.68\u2009mm<jats:sup>3<\/jats:sup>) (<jats:italic>p<\/jats:italic>\u2009=\u20090.008).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Implantation of BMP\u20107 gene activated fat tissue fragments can elicit regeneration of large bone defects in rats and could become a clinically expeditious strategy for <jats:italic>in vivo<\/jats:italic> bone tissue engineering. However, gene expression must be improved in order to reliably induce osseous bridging of critical\u2010size bone defects. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2892","type":"journal-article","created":{"date-parts":[[2016,7,4]],"date-time":"2016-07-04T08:19:01Z","timestamp":1467620341000},"page":"199-207","source":"Crossref","is-referenced-by-count":10,"title":["An expedited approach for sustained delivery of bone morphogenetic protein\u20107 to bone defects using gene activated fragments of subcutaneous fat"],"prefix":"10.1002","volume":"18","author":[{"given":"Volker M.","family":"Betz","sequence":"first","affiliation":[{"name":"Department of Trauma and Reconstructive Surgery and Center for Translational Bone, Joint and Soft Tissue Research University Hospital Carl Gustav Carus Dresden, TU Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver B.","family":"Betz","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Physical Medicine and Rehabilitation University Hospital Grosshadern, Ludwig\u2010Maximilians\u2010University Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Rosin","sequence":"additional","affiliation":[{"name":"Department of Trauma and Reconstructive Surgery and Center for Translational Bone, Joint and Soft Tissue Research University Hospital Carl Gustav Carus Dresden, TU Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Keller","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Physical Medicine and Rehabilitation University Hospital Grosshadern, Ludwig\u2010Maximilians\u2010University Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Thirion","sequence":"additional","affiliation":[{"name":"Sirion Biotech GmbH  Martinsried 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