{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:59:05Z","timestamp":1759777145810,"version":"3.41.2"},"reference-count":54,"publisher":"Wiley","issue":"9-10","license":[{"start":{"date-parts":[[2017,9,22]],"date-time":"2017-09-22T00:00:00Z","timestamp":1506038400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["0315577C (QuReGe)"],"award-info":[{"award-number":["0315577C (QuReGe)"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previously published data indicate that BMP\u20102 gene activated muscle tissue grafts can repair large bone defects in rats. This innovative abbreviated <jats:italic>ex vivo<\/jats:italic> gene therapy is appealing because it does not require elaborative and time\u2010consuming extraction and expansion of cells. Hence, in the present study, we evaluated the potential of this expedited tissue engineering approach for regenerating osteochondral defects in rabbits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Autologous muscle tissue grafts from female White New Zealand rabbits were directly transduced with an adenoviral BMP\u20102 vector or remained unmodified. Osteochondral defects in the medial condyle of rabbit knees were treated with either BMP\u20102 activated muscle tissue implants or unmodified muscle tissue or remained empty. After 13\u00a0weeks, repair of osteochondral defects was examined by biomechanical indentation testing and by histology\/imunohistochemistry applying an extended O'Driscoll scoring system and histomorphometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biomechanical investigations revealed a trend towards slightly improved mechanical properties of the group receiving BMP\u20102 activated muscle tissue compared to unmodified muscle treatment and empty defect controls. However, a statistically significant difference was noted only between BMP\u20102 muscle and unmodified muscle treatment. Also, histological evaluation resulted in slightly higher histological scores and improved collagen I\/II ratio without statistical significance in the BMP\u20102 treatment group. Histomorphometry indicated enhanced repair of subchondral bone after treatment with BMP\u20102 muscle, with a significantly larger bone area compared to untreated defects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Gene activated muscle tissue grafts showed potential for osteochondral defect repair. There is room for improvement via the use of appropriate growth factor combinations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2972","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T01:06:43Z","timestamp":1501031203000},"source":"Crossref","is-referenced-by-count":12,"title":["BMP\u20102 gene activated muscle tissue fragments for osteochondral defect regeneration in the rabbit knee"],"prefix":"10.1002","volume":"19","author":[{"given":"Volker M.","family":"Betz","sequence":"first","affiliation":[{"name":"University Center of Orthopaedics and Traumatology and Center for Translational Bone, Joint and Soft Tissue Research University Hospital Carl Gustav Carus Dresden, Technical University Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Keller","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation University Hospital Grosshadern, Ludwig\u2010Maximilians\u2010University Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Foehr","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Sportsorthopaedics Klinikum rechts der Isar, Technical University of Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Thirion","sequence":"additional","affiliation":[{"name":"Sirion Biotech GmbH  Martinsried Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Salomon","sequence":"additional","affiliation":[{"name":"Sirion Biotech GmbH  Martinsried Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Rammelt","sequence":"additional","affiliation":[{"name":"University Center of Orthopaedics and Traumatology and Center for Translational Bone, Joint and Soft Tissue Research University Hospital Carl Gustav Carus 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