{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:29:16Z","timestamp":1777555756292,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002867","name":"Chongqing University of Arts and Sciences","doi-asserted-by":"publisher","award":["R2016BX09"],"award-info":[{"award-number":["R2016BX09"]}],"id":[{"id":"10.13039\/501100002867","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007957","name":"Chongqing Municipal Education Commission","doi-asserted-by":"publisher","award":["KJ1711279"],"award-info":[{"award-number":["KJ1711279"]}],"id":[{"id":"10.13039\/501100007957","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neuropathic pain (NP) is a refractory disease in the clinic with a tremendous impact on the quality of life of patients. Gene therapy is a potential strategy for the management of NP. In the present study, we examined the analgesic effect and mechanism of hepatocyte growth factor (HGF) <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the proinflammatroy gene changes in lipopolysaccharide (LPS)\u2010induced microglia BV2 cells with a quantitative real\u2010time polymerase chain reaction of interleukin (IL)\u20101\u03b2, IL\u20106, tumor necrosis factor (TNF)\u2010\u03b1 and inducible nitric oxide synthase (iNOS). Mechanical stimulation tests were performed five times at 5\u2010min intervals to assess pain thresholds using Von Frey Hair in mice following spared nerve injury (SNI). The glial cell activation of spinal cord was examined by western blotting. Statistical significance was determined by a Tukey's test and a paired <jats:italic>t<\/jats:italic>\u2010test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that recombinant human HGF protein suppressed LPS\u2010induced BV2 cell activation <jats:italic>in vitro<\/jats:italic>, marked by the down\u2010regulation of IL\u20101\u03b2, IL\u20106, TNF\u2010\u03b1 and iNOS expression, as well as decrease of nitric oxide production. Moreover, intrathecal injection of naked plasmid encoding HGF gene (pUDK\u2010HGF) significantly attenuated SNI\u2010induced pain behaviors in mice by direct inhibition of spinal cord microglia and astrocyte activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results of the present study indicate that pUDK\u2010HGF can reduce cytotoxicity products released from activated glial cells, which may provide a promising therapeutic strategy for treating NP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.2994","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T18:37:14Z","timestamp":1508783834000},"source":"Crossref","is-referenced-by-count":9,"title":["Gene therapy for neuropathic pain induced by spared nerve injury with naked plasmid encoding hepatocyte growth factor"],"prefix":"10.1002","volume":"19","author":[{"given":"Chunsheng","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Experimental Hematology Beijing Institute of Radiation Medicine  Beijing China"},{"name":"International Academy of Targeted Therapeutics and Innovation Chongqing University of Arts and Sciences  Chongqing China"},{"name":"College of Life Science and Bioengineering Beijing University of Technology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Experimental Hematology Beijing Institute of Radiation Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Experimental Hematology Beijing Institute of Radiation Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufang","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Experimental Hematology Beijing Institute of Radiation Medicine  Beijing 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