{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T13:15:03Z","timestamp":1769865303964,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2019,1,22]],"date-time":"2019-01-22T00:00:00Z","timestamp":1548115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2017BH099"],"award-info":[{"award-number":["ZR2017BH099"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adiponectin (APN) has been reported to promote bone formation. However, it is difficult to utilize a conventional method that administers sufficient APN to the implant site. The present study investigated the efficacy of an APN transgene to accelerate the implant osseointegration in ovariectomized (OVX) rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>In vitro<\/jats:italic>, bone marrow stromal cells were transduced with reconstructed adenovirus (Ad\u2010APN\u2010EGFP) and osteoclast precursor RAW264.7 cells were co\u2010cultured with the conditioned medium secreted by transduced bone marrow stromal cells. Tartrate\u2010resistant acid phosphatase staining and bone slice resorption assay were performed to evaluate the activity of osteoclastogenesis. <jats:italic>In vivo<\/jats:italic>, Ad\u2010APN\u2010EGFP was administered into the bone defect prior to implant placement in OVX rats. At 7 and 28\u00a0days post implantation, the femurs were harvested and prepared for a real\u2010time reverse transcriptase\u2010polymerase chain reaction, hemotoxylin and eosin staining, immunohistochemical staining, micro\u2010computed tomography analysis and biomechanical testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed the formation and function of osteoclasts were significantly suppressed <jats:italic>in vitro<\/jats:italic>. Successful transgene expression was confirmed, and a significant increase of OCN, Runx2 and ALP expression was detected in the Ad\u2010APN\u2010EGFP group <jats:italic>in vivo<\/jats:italic>. Interestingly, we also found that the overexpression of APN decreased the expression level of potent adipogenic transcription factors such as PPAR\u03b32 and C\/EBP\u2010\u03b1. At 28\u00a0days after implantation, the Ad\u2010APN\u2010EGFP group revealed a significantly increased osseointegration and implant stability in OVX rats compared to the control groups (Ad\u2010EGFP and PBS groups).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>APN via direct adenovirus\u2010mediated gene transfer could ameliorate osseointegration surrounding titanium implants in OVX\u2010related osteoporosis rats. Furthermore, it may be an effective strategy for promoting bone regeneration under osteoporotic conditions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3069","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T21:44:25Z","timestamp":1546551865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Adenoviral delivery of adiponectin ameliorates osteogenesis around implants in ovariectomized rats"],"prefix":"10.1002","volume":"21","author":[{"given":"Guozhu","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Stomatology Shandong Provincial Hospital affiliated to Shandong University  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanghang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery West China Hospital of Stomatology, Sichuan University  Chengdu China"},{"name":"Division of Oral Biology Tufts University School of Dental Medicine  Boston MA 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