{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:30:34Z","timestamp":1779341434963,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The present study aimed to investigate the association of several single nucleotide polymorphisms (SNPs) within the Toll\u2010like receptor 4 (<jats:italic>TLR4<\/jats:italic>) gene and additional gene\u2013environment interaction with primary open angle glaucoma (POAG) risk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tests for Hardy\u2013Weinberg equilibrium in controls and haplotype analysis were performed using SNPstats (https:\/\/www.snpstats.net). Generalized multifactor dimensionality reduction (GMDR) was performed to test the interaction effects among four SNPs within the <jats:italic>TLR4<\/jats:italic> gene and environmental factors. Logistic regression was performed to calculate the odds ratios (ORs) (95% confidence interval [CI]) for association between four SNPs within the <jats:italic>TLR4<\/jats:italic> gene and POAG risk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The POAG risk was significantly higher in carriers with the T allele of <jats:bold>rs4986791<\/jats:bold> and the T allele of <jats:bold>rs2149356<\/jats:bold> within the <jats:italic>TLR4<\/jats:italic> gene than in those with the wild\u2010type genotype, adjusted ORs (95% CI) were 1.65 (1.23\u20132.12) and 1.70 (1.16\u20132.31). The GMDR model suggested a significant two\u2010locus model (<jats:italic>p<\/jats:italic> = 0.0010) involving rs2149356 and alcohol drinking. Alcohol drinkers with the rs2149356\u2010GT+TT genotype within the <jats:italic>TLR4<\/jats:italic> gene have the highest POAG risk compared to never alcohol drinkers with the rs2149356\u2010GG genotype (OR = 2.62; 95% CI = 1.48\u20133.78) after covariates adjustment. However, the study did not find a significant any\u2010locus model involving SNP and smoking. In all samples, the haplotype rs2149356\u2010<jats:bold>G\u2010<\/jats:bold>rs7873784\u2010<jats:bold>C<\/jats:bold> was observed most frequently in two groups (47.47% and 48.21% for the POAG patients and controls, respectively). The results also indicated that no significant haplotype was associated with POAG risk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The minor alleles of <jats:bold>rs4986791<\/jats:bold> and <jats:bold>rs2149356<\/jats:bold> within the <jats:italic>TLR4<\/jats:italic> gene, as well as interaction between rs2149356 and alcohol drinking, were associated with an increased POAG risk.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3139","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T11:35:31Z","timestamp":1573126531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Association of single\u2010nucleotide polymorphisms in TLR4 gene and gene\u2013environment interaction with primary open angle glaucoma in a Chinese northern population"],"prefix":"10.1002","volume":"22","author":[{"given":"Huijuan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Baodi Clinical College of Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shixin","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Baodi Clinical College of Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Baodi Clinical College of Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuirong","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Baodi Clinical College of Tianjin Medical University  Tianjin 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