{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T06:25:40Z","timestamp":1762928740624,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302148","31500840"],"award-info":[{"award-number":["31302148","31500840"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Intracellular bacteria, especially <jats:styled-content style=\"fixed-case\"><jats:italic>Mycobacterium tuberculosis<\/jats:italic><\/jats:styled-content>, are important pathogenic microorganisms that endanger human health. Purified and synthesized cecropin A\u2010magainin 2 (CAMA\u2010syn) can exhibit a higher antibacterial activity and lower cytotoxicity. To enhance such antimicrobial potential, it would be desirable to deliver CAMA\u2010syn expressed in lung epithelial cells by an adenovirus vector using gene therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A549 cells <jats:italic>in vitro<\/jats:italic> and lung epithelial cells <jats:italic>in vivo<\/jats:italic> were used to express CAMA\u2010syn by transducing recombinant adenovirus Ad\u2010SPC\u2010CAMA\/GFP, and the expression of CAMA\u2010syn was determined by a reverse transcriptase\u2010polymerase reaction (RT\u2010PCR) and immunofluorescence. The antimicrobial activity in cells was investigated by colony\u2010forming rate and growth curve. Forty Kunming mice of a Bacillus Calmette\u2010Guerin (BCG) infection animal model were randomly divided into three groups: adenoviruses delivery of Ad\u2010SPC\u2010CAMA\/GFP, Ad\u2010CMV\u2010CAMA\/GFP and empty\u2010virus Ad\u2010CMV\u2010GFP. The expression of CAMA\u2010syn in mice was confirmed by RT\u2010PCR and immunofluorescence. After tracheal injection of adenoviral vector for 3 days, lungs from the mouse model were extracted and homogenized for detection of colony\u2010forming efficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CAMA\u2010syn expressed in lung epithelial cells A549 conferred antimicrobial activity against a series of bacteria, including <jats:italic>Salmonella abortusovis<\/jats:italic> and BCG. The results obtained <jats:italic>in vivo<\/jats:italic> showed that the colony\u2010forming rate of Ad\u2010SPC\u2010CAMA\/GFP (74.54%) and Ad\u2010CMV\u2010CAMA\/GFP (62.31%) transduced into mice was significantly lower than that of the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Lung epithelial\u2010specific expression of antimicrobial peptide CAMA\u2010syn mediated by adenovirus suppressed the growth of intracellular bacteria, providing a promising approach for the control of refractory intracellular infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3149","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T19:39:02Z","timestamp":1574797142000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Antimicrobial peptide CAMA\u2010syn expressed in pulmonary epithelium by recombination adenovirus inhibited the growth of intracellular 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