{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:02:12Z","timestamp":1793091732579,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,23]],"date-time":"2020-08-23T00:00:00Z","timestamp":1598140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The present study aimed to explore the functions and molecular mechanisms of miR\u2010511 in breast cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was used to detect miR\u2010511 levels in breast cancer tissues; a chi\u2010squared test was used to analyze the relationship between miR\u2010511 expression level and pathological parameters of breast cancer patients; the proliferation of breast cancer cell lines MDA\u2010MB\u2010231 and MCF\u20107 was determined by the cell counting kit\u20108 (CCK\u20108) assay; migration was determined by scratch wound healing assay and transwell assay; TargetScan was used to predict the binding site between the 3'\u2010untranslated region (3'\u2010UTR) of fibroblast growth factor 4 (FGF4) and miR\u2010511; and qRT\u2010PCR, western blot and a luciferase reporter gene assay were conducted to further validate the targeting relationship between miR\u2010511 and FGF4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression level of miR\u2010511 was lower in breast cancer tissues than that in adjacent normal tissues. Low expression of miR\u2010511 was associated with larger tumor size, lymph node metastasis and short survival time. <jats:italic>In vitro<\/jats:italic> experiments showed that miR\u2010511 modulated the proliferation and metastasis of breast cancer cells. It was also confirmed that miR\u2010511 directly targeted 3'\u2010UTR of FGF4 and reduced its expression, and FGF4 overexpression reversed the effect of miR\u2010511 on the malignant phenotypes of breast cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results obtained in the present study demonstrate that miR\u2010511 inhibits breast cancer proliferation and metastasis by down\u2010regulating FGF4 expression, which may be helpful in the development of new treatment strategies for breast cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3168","type":"journal-article","created":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T18:14:10Z","timestamp":1580926450000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["miR\u2010511 inhibits proliferation and metastasis of breast cancer cells by targeting 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