{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T15:56:53Z","timestamp":1768838213907,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ganzhou City of China","award":["2013-075"],"award-info":[{"award-number":["2013-075"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As a potential anti\u2010arthritic agent, Andrographolide (And) is capable of promoting chondrocyte proliferation and preventing apoptosis in pathologic condition. The present study aimed to explore the roles of And in <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> models of osteoarthritis (OA), as well as its underlying molecular mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An OA mouse model was established using anterior cruciate ligament transection operation on the left knee joint. The pathological changes of articular cartilage were assessed using safranin O staining. Chondrocyte proliferation and apoptosis were measured using cell a counting kit\u20108 assay and flow cytometry. Bioinformatics algorithms and a luciferase reporter assay were used to evaluate matrix metalloproteinase13 (MMP13) as a direct target of miR\u201027\u20103p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>And had the ability to prevent catabolism and facilitate anabolism of articular cartilage in an experimental OA model in mice. In addition, And alleviated chondrocyte apoptosis in <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> models of OA. We also found that both up\u2010regulation of MMP13 and down\u2010regulation of miR\u201027\u20103p in the proximal tibia of OA mice and interleukin (IL)\u20101\u03b2\u2010stimulated chondrocytes were reversed by And administration simultaneously. MMP13 was validated as direct target of miR\u201027\u20103p and could be suppressed by overexpression of miR\u201027\u20103p in mouse chondrocyte. Furthermore, overexpression of miR\u201027\u20103p or MMP13 loss\u2010of\u2010function in chondrocytes could alleviate IL\u20101\u03b2\u2010induced apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicated that miR\u201027\u20103p\/MMP13 signaling axis might be a potential therapeutic target of And for preventing the progression of OA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3187","type":"journal-article","created":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T19:00:16Z","timestamp":1584730816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Andrographolide mitigates cartilage damage via miR\u201027\u20103p\u2010modulated matrix metalloproteinase13 repression"],"prefix":"10.1002","volume":"22","author":[{"given":"Shaojian","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Sports Medical Ganzhou People's Hospital &amp; the 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