{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:28:58Z","timestamp":1787063338181,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T00:00:00Z","timestamp":1590019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\u03b2\u2010ecdysone (\u03b2Ecd) has numerous pharmacological effects, although its role in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) has not yet been explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In cell experiments, BMSCs were induced to differentiate by osteogenic induction medium (OIM) or \u03b2Ecd. In animal experiments, an osteonecrosis of the femoral head (ONFH) rat model was established using lipopolysaccharide plus methylprednisolone and treating the rats with \u03b2Ecd. The osteogenic differentiation capacity of human BMSCs (hBMSCs) was analyzed by alkaline phosphatase and alizarin red S staining. Histopathological changes in rat femoral head tissues were observed by hematoxylin and eosin staining. The expression levels of RUNX2, COL1A1, OCN and phosphorylated Akt in BMSCs from rat femoral head tissues were measured by a quantitative real\u2010time polymerase chain reaction or western blot analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Alkaline phosphatase activity and calcium nodules in the \u03b2Ecd\u2010treated BMSC group dose\u2010dependently increased compared to those in the control and OIM groups. The hematoxylin and eosin staining results indicated that femoral head tissues of ONFH rats showed typical osteonecrosis, which could be ameliorated by \u03b2Ecd. Western blot, quantitative real\u2010time polymerase chain reaction and immunohistochemistry assays demonstrated that the expression levels of RUNX2, COL1A1 and OCN in hBMSCs and femoral head tissue models were obviously increased after \u03b2Ecd treatment, and phosphoinositide 3\u2010kinase and Akt phosphorylation were also increased.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\u03b2Ecd may be beneficial for the recovery of ONFH patients by accelerating osteogenic differentiation of BMSCs, which may be a novel therapy for related diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.3207","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T05:32:35Z","timestamp":1588224755000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["\u03b2\u2010ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6421-0240","authenticated-orcid":false,"given":"Wei\u2010Li","family":"You","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First People's Hospital of Lianyungang  Lianyungang Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng\u2010Long","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Xinghua City People's Hospitafl  Xinghua City Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.O.00071"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55188-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8759642"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.12.116"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-019-1243-5"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-019-1399-z"},{"key":"e_1_2_10_8_1","first-page":"4984","article-title":"Cnidium lactone stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via BMP\u20102\/smad\u2010signaling cascades mediated by estrogen receptor","volume":"11","author":"Wang Z","year":"2019","journal-title":"Am J Trans Res"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2019.06.016"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29161"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153800"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-015-4246-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-019-1440-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.03.002"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S178698"},{"key":"e_1_2_10_16_1","first-page":"1591","article-title":"betaEcdysterone promotes autophagy and inhibits apoptosis in osteoporotic rats","volume":"17","author":"Tang YH","year":"2018","journal-title":"Mol Med Rep"},{"key":"e_1_2_10_17_1","first-page":"489","article-title":"Beta\u2010Ecdysterone protects SH\u2010SY5Y cells against beta\u2010amyloid\u2010induced apoptosis via c\u2010Jun N\u2010terminal kinase\u2010 and Akt\u2010associated complementary pathways. Laboratory investigation","volume":"98","author":"Xu T","year":"2018","journal-title":"J Tech Methods Pathol"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.01.001"},{"key":"e_1_2_10_19_1","first-page":"208","article-title":"Dihydrotestosterone and 17\u2010estradiol enhancement of in vitro osteogenic differentiation of castrated male rat bone marrow mesenchymal stem cells (rBMMSCs)","volume":"13","author":"Abo\u2010Aziza F","year":"2019","journal-title":"International Journal of Hematology\u2010Oncology and Stem Cell Research"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","unstructured":"LiH YangC.Arctigenin promotes bone formation involving PI3K\/Akt\/PPARgamma signaling pathway. Chemical biology & drug design.2019;95:451\u2010459.","DOI":"10.1111\/cbdd.13659"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.2245"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v028a19"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1168-2"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2321"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06900"},{"key":"e_1_2_10_26_1","first-page":"456","article-title":"Effect of turkesterone and nerobol on the activity of the protein synthesizing system of mouse liver","volume":"24","author":"Syrov VN","year":"1978","journal-title":"Voprosy Meditsinskoi Khimii"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.11.061"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjgm.3207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.3207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jgm.3207","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.3207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:52:41Z","timestamp":1693875161000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.3207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,21]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/jgm.3207"],"URL":"https:\/\/doi.org\/10.1002\/jgm.3207","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"value":"1099-498X","type":"print"},{"value":"1521-2254","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,21]]},"assertion":[{"value":"2020-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3207"}}