{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:31:49Z","timestamp":1791311509802,"version":"4.3.1"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,9,27]],"date-time":"2002-09-27T00:00:00Z","timestamp":1033084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Gene therapy is very promising in the treatment of rheumatoid arthritis (RA). Electrotransfer is a recent method reported to enhance <jats:italic>in vivo<\/jats:italic> intramuscular DNA transfection. Interleukin\u201010 (IL\u201010) has antiinflammatory effects in RA and in collagen\u2010induced arthritis (CIA), a murine model of RA. In order to improve our strategy of gene therapy, we used electrotransfer to enhance penetration into skeletal muscle with CIA of plasmids encoding IL\u201010.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CIA was induced in DBA\/1 mice by immunization with bovine type II collagen. Injection into the tibial cranial muscle of low\u2010dose (200 ng) pCOR plasmid encoding murine IL\u201010 (pCOR\u2010CMV\u2010mIL\u201010) was immediately followed by application of square\u2010wave electric pulses (8 pulses of 200V\/cm, 20 ms duration at 2 Hz). Control groups received empty plasmid or saline before electrotransfer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When electrotransfer was performed twice on days 10 and 25 postimmunization, CIA was significantly delayed (<jats:italic>P<\/jats:italic> &lt; 0.05) and attenuated (<jats:italic>P<\/jats:italic> &lt; 0.001) in groups treated by electrotransfer or pCOR\u2010CMV\u2010mIL\u201010 plasmid vs. control groups. When electrotransfer of pCOR\u2010CMV\u2010mIL\u201010 plasmid was performed on days 25 and 40 postimmunization, at disease onset, the clinical severity of CIA was reduced (<jats:italic>P<\/jats:italic> &lt; 0.05). All groups which had been electrotransferred early or late by pCOR\u2010CMV\u2010mIL\u201010 plasmid showed suppression of histological signs of arthritis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken together, these data indicate that administration of an antiinflammatory plasmid\u2010born gene by electrotransfer of naked DNA is effective <jats:italic>in vivo<\/jats:italic> in an arthritis model. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.321","type":"journal-article","created":{"date-parts":[[2003,1,22]],"date-time":"2003-01-22T22:11:32Z","timestamp":1043273492000},"page":"164-171","source":"Crossref","is-referenced-by-count":48,"title":["Efficacy of interleukin\u201010 gene electrotransfer into skeletal muscle in mice with collagen\u2010induced 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