{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:28:42Z","timestamp":1793824122887,"version":"4.6.1"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2020YFC2002800"],"award-info":[{"award-number":["2020YFC2002800"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoarthritis (OA) is a degenerative disease characterized by cartilage damage. We aimed to improve the understanding of the protective mechanism of synovial mesenchymal stem cell (SMSC)\u2010derived extracellular vesicles (EVs) in cartilage damage of OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SMSCs and SMSC\u2010EVs were isolated from synovial biopsies of patients without OA and then identified. The pathological microenvironment of chondrocytes in OA was simulated by inducing SW1353 cells with interleukin (IL)\u20101\u03b2, followed by SMSC\u2010EV treatment to assess SW1353 cell proliferation, apoptosis and inflammation. Endocytosis of Dil\u2010labeled EVs by SW1353 cells was observed. microRNA (miR)\u201026a\u20105p expression in EVs and EV\u2010treated SW1353 cells was assessed. The effect of miR\u201026a\u20105p was evaluated after it was down\u2010regulated in SMSCs, followed by extraction of EVs, which acted on SW1353 cells. The target relationship of miR\u201026a\u20105p and phosphatase and tensin homologue (PTEN) was predicted and confirmed. The role of PTEN in OA was evaluated after it was overexpressed. Functional assays were implemented <jats:italic>in vivo<\/jats:italic> to certify the role of SMSC\u2010EVs in OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SMSC\u2010EVs enhanced IL\u20101\u03b2\u2010induced SW1353 cell proliferation, whereas they inhibited apoptosis and inflammation. EVs were endocytosed by SW1353 cells and delivered miR\u201026a\u20105p into SW1353 cells to overexpress miR\u201026a\u20105p. Down\u2010regulation of miR\u201026a\u20105p in EVs attenuated the protection of EVs against IL\u20101\u03b2\u2010induced cell damage. miR\u201026a\u20105p targeted PTEN, for which overexpression spoiled the protection of EVs against IL\u20101\u03b2\u2010induced cell damage. SMSC\u2010EVs carrying miR\u201026a\u20105p repaired cartilage damage of OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SMSC\u2010EVs carried miR\u201026a\u20105p into chondrocytes to up\u2010regulate miR\u201026a\u20105p and inhibit PTEN, thereby inhibiting apoptosis and inflammation and ameliorating cartilage damage of OA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3379","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T10:44:45Z","timestamp":1627037085000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Synovial mesenchymal stem cell\u2010derived extracellular vesicles containing microRN555A\u201026a\u20105p ameliorate cartilage damage of osteoarthritis"],"prefix":"10.1002","volume":"23","author":[{"given":"Laiya","family":"Lu","sequence":"first","affiliation":[{"name":"Shanghai East Hospital Tongji University, School of Medicine  Shanghai 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