{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T03:00:57Z","timestamp":1769223657687,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circular RNA itchy E3 ubiquitin protein ligase (circ\u2010ITCH) has previously been reported to play a key role in carcinogenesis. Nevertheless, the role of circ\u2010ITCH in nasopharyngeal carcinoma (NPC) remains to be explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gene expression analysis was performed using a quantitative real\u2010time polymerase chain reaction, western blotting and immunohistochemistry. The role of circ\u2010ITCH in NPC was explored using 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl tetrazolium bromide, colony formation, transwell invasion, scratch healing and xenograft tumor assays. Furthermore, luciferase reporter assay was carried out to assess the interactions among circ\u2010ITCH, microRNA\u2010214 (miR\u2010214) and phosphatase and tensin homolog (PTEN).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The levels of circ\u2010ITCH and PTEN were decreased, whereas the level of miR\u2010214 was increased in NPC tissues collected from 28 subjects compared to normal nasopharynx tissues collected from 15 subjects. Moreover, a negative correlation between circ\u2010ITCH and miR\u2010214 expression and a positive correlation between circ\u2010ITCH and PTEN expression were observed in NPC tissues. Downregulation of circ\u2010ITCH expression was also observed in NPC cell lines. In addition, upregulation of circ\u2010ITCH markedly inhibited NPC cell proliferation, migration and invasion. Furthermore, circ\u2010ITCH was confirmed to exert its function by sponging miR\u2010214. PTEN was found to be a direct target gene of miR\u2010214 and its expression was negatively correlated with miR\u2010214 expression in NPC tissues. Moreover, our results showed that the circ\u2010ITCH\/miR\u2010214 axis regulated NPC proliferation, migration and invasion through regulating the expression of PTEN. Upregulation of circ\u2010ITCH or PTEN blocked miR\u2010214\u2010mediated promotion of NPC tumorigenesis <jats:italic>in vitro<\/jats:italic>. Additionally, upregulation of circ\u2010ITCH also suppressed NPC tumorigenesis <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study demonstrated that circ\u2010ITCH suppressed NPC tumorigenesis by upregulating PTEN expression through interacting with miR\u2010214, thus proposing a novel mechanism for NPC inhibition.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3391","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T02:13:22Z","timestamp":1633659202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Circular RNA ITCH attenuates the progression of nasopharyngeal carcinoma by inducing PTEN upregulation via miR\u2010214"],"prefix":"10.1002","volume":"24","author":[{"given":"Liuzhong","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Pharyngolaryngology Head and Neck Surgery, Otorhinolaryngology Hospital The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhong","family":"Sang","sequence":"additional","affiliation":[{"name":"Division of Pharyngolaryngology Head and Neck Surgery, Otorhinolaryngology Hospital The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Pharyngolaryngology Head and Neck Surgery, Otorhinolaryngology Hospital The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Pharyngolaryngology Head and Neck Surgery, Otorhinolaryngology Hospital The First Affiliated Hospital of Zhengzhou 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