{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:01:52Z","timestamp":1776355312758,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:00:00Z","timestamp":1646438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Inadequate trophoblast invasion is associated with preeclampsia (PE). Ankyrin repeat domain protein 37 (ANKRD37) has been reported to be abnormally expressed in PE placentas. However, the role of ANKRD37 in trophoblasts has not been investigated. We aimed to determine the functions of ANKRD37 in PE and to explore the molecular mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, fluorescence <jats:italic>in situ<\/jats:italic> hybridization, immunohistochemistry, Western blotting and quantitative real\u2010time polymerase chain reaction were used to detect protein and mRNA expression levels. Cell counting kit\u20108 assay, 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine assay, flow cytometry, wound healing assay, transwell assay and RNA sequencing were performed to investigate the role of ANKRD37 and the underlying mechanism in HTR8\/SVneo and JEG\u20103 cells, and extravillous explant cultures were used to evaluate the migration and invasion abilities of extravillous cytotrophoblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that ANKRD37 expression was upregulated in PE placentas compared to normal pregnancy placentas. ANKRD37 knockdown enhanced trophoblast migration and invasion, promoted extravillous explant outgrowth, and regulated the expression of key invasion proteins, whereas ANKRD37 overexpression exerted the opposite effects. RNA sequencing indicated that nuclear factor\u2010kappa B (NF\u2010\u03baB) was the potential downstream pathway of ANKRD37, which was confirmed by the change in p\u2010p65 and p\u2010I\u03baB\u03b1 expression in JEG\u20103 and HTR8\/SVneo cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that high expression of ANKRD37 inhibits trophoblast cell migration and invasion possibly via the NF\u2010\u03baB pathway, and may be related to the development of PE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3416","type":"journal-article","created":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T13:05:00Z","timestamp":1645880700000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["ANKRD37 inhibits trophoblast migration and invasion by regulating the NF\u2010\u03baB pathway in preeclampsia"],"prefix":"10.1002","volume":"24","author":[{"given":"Wang","family":"Tan","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijia","family":"Fu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center The First Affiliated Hospital of Chongqing Medical University  Chongqing 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