{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:37:04Z","timestamp":1789835824728,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["19ae0201007h0002"],"award-info":[{"award-number":["19ae0201007h0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["20ae0201007h0003"],"award-info":[{"award-number":["20ae0201007h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The delivery of adeno\u2010associated virus (AAV) vectors via the cerebrospinal fluid (CSF) has emerged as a valuable method for widespread transduction in the central nervous system. Although infusion into the cerebral ventricles is a common protocol in preclinical studies of small animals, the cisterna magna has been recognized as an alternative target for clinical studies because it can be reached in a less invasive manner using an intrathecal catheter via the subarachnoid space from a lumbar puncture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated the early distribution of fluorine\u201018\u2010labeled AAV9 vectors infused into the lateral ventricle or cisterna magna of four non\u2010human primates using positron emission tomography. The expression of the green fluorescent protein was immunohistochemically determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In both approaches, the labeled vectors diffused into the broad arachnoid space around the brain stem and cervical spinal cord within 30\u2009min. Both infusion routes efficiently transduced neurons in the cervical spinal cord.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>For gene therapy that primarily targets the cervical spinal cord and brainstem, such as amyotrophic lateral sclerosis, cisterna magna infusion would be a feasible and effective administration method.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.3457","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T10:54:30Z","timestamp":1666608870000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Early distribution of\n                    <sup>18<\/sup>\n                    F\u2010labeled AAV9 vectors in the cerebrospinal fluid after intracerebroventricular or intracisternal magna infusion in non\u2010human primates"],"prefix":"10.1002","volume":"25","author":[{"given":"Shinichi","family":"Kumagai","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Jichi Medical University  Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Jichi Medical University  Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniko","family":"Shimazaki","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Jichi Medical University  Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeharu","family":"Kakiuchi","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K.  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Harada","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K.  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Ohba","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K.  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