{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:39:07Z","timestamp":1790786347432,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T00:00:00Z","timestamp":1684195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900667"],"award-info":[{"award-number":["81900667"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Peritoneal fibrosis is a common complication of peritoneal dialysis, which may lead to ultrafiltration failure and ultimately treatment discontinuation. LncRNAs participate in many biological processes during tumorigenesis. We investigated the role of AK142426 in peritoneal fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The AK142426 level in peritoneal dialysis (PD) fluid was detected by quantitative real\u2010time\u2010PCR assay. The M2 macrophage distribution was determined by flow cytometry. The inflammatory cytokines of TNF\u2010<jats:italic>\u03b1<\/jats:italic> and TGF\u2010<jats:italic>\u03b2<\/jats:italic>1 were measured by ELISA assay. The direct interaction between AK142426 and c\u2010Jun was evaluated by RNA pull\u2010down assay. In addition, the c\u2010Jun and fibrosis related proteins were assessed by western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PD\u2010induced peritoneal fibrosis mouse model was successfully established. More importantly, PD treatment induced M2 macrophage polarization and the inflammation in PD fluid, which might be associated with exosome transmission. Fortunately, AK142426 was observed to be upregulated in PD fluid. Mechanically, knockdown of AK142426 suppressed M2 macrophage polarization and inflammation. Furthermore, AK142426 could upregulate c\u2010Jun through binding c\u2010Jun protein. In rescue experiments, overexpression of c\u2010Jun could partially abolish the inhibitory effect of sh\u2010AK142426 on the activation of M2 macrophages and inflammation. Consistently, knockdown of AK142426 alleviated peritoneal fibrosis <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated that knockdown of AK142426 suppressed M2 macrophage polarization and inflammation in peritoneal fibrosis via binding to c\u2010Jun, suggesting that AK142426 might be a promising therapeutic target for patients of peritoneal fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3524","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T05:45:38Z","timestamp":1684302338000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Knockdown of AK142426 suppresses M2 macrophage polarization and inflammation in peritoneal fibrosis via binding to c\u2010Jun"],"prefix":"10.1002","volume":"25","author":[{"given":"Qiuyuan","family":"Shao","sequence":"first","affiliation":[{"name":"Department of 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