{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T10:55:15Z","timestamp":1769252115528,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T00:00:00Z","timestamp":1683936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["82002098"],"award-info":[{"award-number":["82002098"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ31122"],"award-info":[{"award-number":["2021JJ31122"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ31001"],"award-info":[{"award-number":["2021JJ31001"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Complex interactions in the tumor microenvironment (TME) between bladder cancer (BLCA) and immune cells are critical for cancer progression. However, studies of neutrophil extracellular trap\u2010associated long non\u2010coding RNAs (NET\u2010lncRNAs) in the TME of BLCA have not been reported. This study aims to screen for NET\u2010lncRNAs in BLCA and to preliminarily explore their effects on BLCA development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The correlation of NET\u2010related gene sets, which were identified from the cancer genome atlas (TCGA) BLCA datasets, with lncRNAs was analyzed and the prognosis\u2010related genes were identified through random forest analysis. The least absolute shrinkage and selection operator (LASSO) model was utilized to obtain prognostic risk scores for NET\u2010lncRNAs (NET\u2010Score). We collected clinical BLCA samples, as well as SV\u2010HUC\u20101 and BLCA cells, to validate the expression of NET\u2010lncRNAs. Survival and independent prognostic analysis were performed. In J82 and UM\u2010UC\u20103 cells, after NKILA expression was inhibited, cell proliferation and apoptosis levels were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NET\u2010related gene sets mainly included CREB5, MMP9, PADI4, CRISPLD2, CD93, DYSF, MAPK3, TECPR2, MAPK1 and PIK3CA. Then, four NET\u2010lncRNAs, MAP 3\u00a0K4\u2010AS1, MIR100HG, NKILA and THY1\u2010AS1, were identified. NET\u2010Score had the highest hazard ratio for BLCA. An elevated NET\u2010Score was linked to a significant increase in immune cell infiltration and copy number variation, as well as a notable decrease in survival rate and drug sensitivity. NET\u2010lncRNA\u2010related genes were mainly enriched in the pathways of angiogenesis, immune response, cell cycle and T cell activation. MAP 3\u00a0K4\u2010AS1, MIR100HG, NKILA and THY1\u2010AS1 expressions were significantly increased in BLCA tissues. Compared with SV\u2010HUC\u20101 cells, NKILA expression was elevated in J82 and UM\u2010UC\u20103 cells. Inhibition of NKILA expression inhibited the proliferation and promoted apoptosis of J82 and UM\u2010UC\u20103 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Several NET\u2010lncRNAs, including MAP 3\u00a0K4\u2010AS1, MIR100HG, NKILA and THY1\u2010AS1, were successfully screened in the BLCA. The NET\u2010Score was an independent prognostic factor for BLCA. In addition, inhibition of NKILA expression suppressed BLCA cell development. The above NET\u2010lncRNAs could serve as potential prognostic markers and targets in BLCA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3525","type":"journal-article","created":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T11:15:45Z","timestamp":1683976545000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prognostic analysis and validation of lncRNAs in bladder cancer on the basis of neutrophil extracellular traps"],"prefix":"10.1002","volume":"25","author":[{"given":"Lan","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Blood Transfusion, The Third Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan 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