{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:00:23Z","timestamp":1792155623365,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:00:00Z","timestamp":1687219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rheumatoid arthritis (RA) is a multifactorial systemic autoimmune disease characterized by ongoing synovial inflammation, leading to the degradation of cartilage. Cuproptosis, as a newly characterized form of cell death, may influence RA progression by regulating immune cells and chondrocytes. This study sets out to identify the hub cuproptosis\u2010related gene (CRG) associated with the pathogenesis of RA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A series of bioinformatic analyses were performed to evaluate the expression score of CRGs and the immune infiltration landscape between RA and normal samples. The hub gene was screened through the correlation analysis of CRGs, and the interaction network between the hub gene and transcription factors (TFs) was constructed. Finally, the hub gene was validated through quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) of patient samples and cell experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Drolipoamide <jats:italic>S<\/jats:italic>\u2010acetyltransferase (<jats:italic>DLAT<\/jats:italic>) was screened as the hub gene. Correlation analysis between the hub gene and immune microenvironment demonstrated that <jats:italic>DLAT<\/jats:italic> had the highest correlation with T follicular helper cells. Eight pairs of <jats:italic>DLAT\u2013<\/jats:italic>TF interaction networks were constructed. Single\u2010cell sequencing showed that CRGs were highly expressed in RA chondrocytes, and chondrocytes could be classified into three different subsets. qRT\u2010PCR was used to validate the above results. <jats:italic>Dlat<\/jats:italic> knockdown in immortalized human chondrocytes led to significantly improved mitochondrial membrane potentials and reduced levels of intracellular reactive oxygen species (ROS), mitochondrial ROS and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study rudimentarily demonstrates the correlation between CRGs and immune cell infiltration in RA. The biomarker <jats:italic>DLAT<\/jats:italic> may provide comprehensive insights into the pathogenesis and drug targets of RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3535","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T12:40:11Z","timestamp":1687264811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identification of a novel cuproptosis\u2010related gene signature for rheumatoid arthritis\u2014A prospective study"],"prefix":"10.1002","volume":"25","author":[{"given":"Han","family":"Hu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Immunity and Inflammation, Institute of Immunology Naval Medical University  Shanghai China"},{"name":"Faculty of Anesthesiology, Changhai Hospital Naval Medical University  Shanghai 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