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Vascular endothelial growth factor\u2010A (VEGFA) was originally identified as a secreted signaling protein and regulator of vascular development and cancer progression. In this study, we aimed to elucidate the molecular mechanisms underlying the regulation of VEGFA by microRNA in UTUC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>VEGFA expression was evaluated by immunohistochemistry in 140 human UTUC tissue samples. Next, we assessed the regulatory relationship between VEGFA and miR\u2010299\u20103p by real\u2010time PCR, western blotting, ELISA and dual\u2010luciferase reporter assays using two UTUC cell lines. The role of miR\u2010299\u20103p\/VEGFA in cell proliferation, motility, invasion, and tube formation was analyzed <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High VEGFA expression was significantly associated with tumor stage, grade, distant metastasis and cancer\u2010related death and correlated with poor progression\u2010free and cancer\u2010specific survival. VEGFA knockdown repressed proliferation, migration, invasion and angiogenesis in UTUC cell lines. miR\u2010299\u20103p significantly reduced VEGFA protein expression and miR\u2010299\u20103p overexpression inhibited VEGFA mRNA and protein expression by directly targeting its 3\u2032\u2010UTR. Functional studies indicated that VEGFA overexpression reversed the miR\u2010299\u20103p\u2010mediated suppression of tumor cell proliferation, migration, invasion and angiogenesis. In addition, miR\u2010299\u20103p\/VEGFA suppressed cellular functions in UTUC by modulating the expression of P18 and cyclin E2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that miR\u2010299\u20103p possibly suppresses UTUC cell proliferation, motility, invasion and angiogenesis via VEGFA. VEGFA may act as a prognostic predictor, and both VEGFA and miR\u2010299\u20103p could be potential therapeutic targets for UTUC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3616","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T05:00:55Z","timestamp":1701752455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["MicroRNA\u2010299\u20103p inhibits cell proliferation, motility, invasion and angiogenesis via VEGFA in upper tract urothelial carcinoma"],"prefix":"10.1002","volume":"26","author":[{"given":"Chien\u2010Shen","family":"Wang","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Kaohsiung Medical University  Kaohsiung Taiwan"},{"name":"Department of Urology Kaohsiung Medical University Hospital  Kaohsiung 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