{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:04:21Z","timestamp":1791313461739,"version":"4.3.1"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,25]],"date-time":"2024-02-25T00:00:00Z","timestamp":1708819200000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003286","name":"Kuwait Foundation for the Advancement of Sciences","doi-asserted-by":"publisher","award":["PR17\u201013MM\u201007"],"award-info":[{"award-number":["PR17\u201013MM\u201007"]}],"id":[{"id":"10.13039\/501100003286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003286","name":"Kuwait Foundation for the Advancement of Sciences","doi-asserted-by":"publisher","award":["RAHM\u20102019\u2010030"],"award-info":[{"award-number":["RAHM\u20102019\u2010030"]}],"id":[{"id":"10.13039\/501100003286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent monogenic renal disease progressing to end\u2010stage renal disease. There is a pressing need for the identification of early ADPKD biomarkers to enable timely intervention and the development of effective therapeutic approaches. Here, we profiled human urinary extracellular vesicles small RNAs by small RNA sequencing in patients with ADPKD and compared their differential expression considering healthy control individuals to identify dysregulated small RNAs and analyze downstream interaction to gain insight about molecular pathophysiology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This is a cross\u2010sectional study where urine samples were collected from a total of 23 PKD1\u2010ADPKD patients and 28 healthy individuals. Urinary extracellular vesicles were purified, and small RNA was isolated and sequenced. Differentially expressed Small RNA were identified and functional enrichment analysis of the critical miRNAs was performed to identify driver genes and affected pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>miR\u2010320b, miR\u2010320c, miR\u2010146a\u20105p, miR\u2010199b\u20103p, miR\u2010671\u20105p, miR\u20101246, miR\u20108485, miR\u20103656, has_piR_020497, has_piR_020496 and has_piR_016271 were significantly upregulated in ADPKD patient urine extracellular vesicles and miRNA\u201029c was significantly downregulated. Five \u2018driver\u2019 target genes (<jats:italic>FBRS<\/jats:italic>, <jats:italic>EDC3<\/jats:italic>, <jats:italic>FMNL3<\/jats:italic>, <jats:italic>CTNNBIP1<\/jats:italic> and <jats:italic>KMT2A<\/jats:italic>) were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The findings of the present study make significant contributions to the understanding of ADPKD pathogenesis and to the identification of novel biomarkers and potential drug targets aimed at slowing disease progression in ADPKD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3674","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T07:19:50Z","timestamp":1708931990000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Global analysis of urinary extracellular vesicle small RNAs in autosomal dominant polycystic kidney disease"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6623-3374","authenticated-orcid":false,"given":"Hamad","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Sciences Center (HSC) Kuwait University  Jabriya Kuwait"},{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute (DDI)  Dasman Kuwait"},{"name":"Division of Nephrology Mubarak Al\u2010Kabeer Hospital, Ministry of Health  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Zubbair","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Abu\u2010Farha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jehad","family":"Abubaker","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preethi","family":"Cherian","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasheeba","family":"Nizam","sequence":"additional","affiliation":[{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sindhu","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousif","family":"Bahbahani","sequence":"additional","affiliation":[{"name":"Division of Nephrology Mubarak Al\u2010Kabeer Hospital, Ministry of Health  Jabriya Kuwait"},{"name":"Medical Division Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Medhat","family":"Naim","sequence":"additional","affiliation":[{"name":"Division of Nephrology Mubarak Al\u2010Kabeer Hospital, Ministry of Health  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajjad","family":"Ahmad","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Al\u2010Sayegh","sequence":"additional","affiliation":[{"name":"Biology Division New York University Abu Dhabi  Abu Dhabi United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangavel Alphonse","family":"Thanaraj","sequence":"additional","affiliation":[{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute (DDI)  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert C. 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