{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:57:34Z","timestamp":1753869454871,"version":"3.41.2"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>With its rapidly increasing incidence and prevalence, ulcerative colitis (UC) has become a major global health challenge. Recent evidence suggests that ferroptosis plays a significant role in the development of UC. However, the relationship between ferroptosis and the progression of UC needs to be extensively studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The differentially expressed genes in UC patients were screened from the GEO database. The ferroptosis\u2010related genes were obtained from FErrDB and GeneCards. The UC subtypes were identified with the R package \u201cCancerSubtype\u201d and evaluated with consensus clustering (CC) to identify gene expression patterns in patients with UC. The key genes were detected with qRT\u2010PCR, Western blot, and immunohistochemistry in vitro and in vivo models. Ferroptosis was identified with western blotting on ferrotic\u2010associated proteins and staining on Fe<jats:sup>2+<\/jats:sup> with commercial FerroOrange kits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Dipeptidyl peptidase 4 (DPP4), also known as CD26, is a potential biomarker for ferroptosis in UC patients. Transcriptome sequencing data showed a positive correlation between decreased DPP4 expression and proinflammatory cytokines such as TNF\u2010\u03b1, IL\u20106, and IL\u2010\u03b2, as well as immune cell infiltration in the colon tissues of UC patients. Furthermore, DPP4 was strongly associated with ferroptosis biomarkers, particularly in Subtype 2 of UC. Interestingly, our study also found that DPP4 expression was significantly reduced in RSL3\u2010treated ferroptotic intestinal epithelial cells, more so than in LPS\u2010treated cell models. Inhibition of DPP4 had a significant impact on the expression of ferroptotic biomarkers. Additionally, DPP4 expression was decreased in the colon tissues of DSS\u2010treated mice, and the ferroptosis inhibitor Ferritin\u20101 effectively counteracted the effects of DSS on immune cell infiltration, colon length, and DPP4 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DPP4 can serve as a biomarker for ferroptosis in the diagnosis and management of UC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.3742","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T03:00:33Z","timestamp":1727665233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dipeptidyl peptidase 4: A predictor of ferroptosis in ulcerative colitis"],"prefix":"10.1002","volume":"26","author":[{"given":"Fuyun","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Pharmacy and Bioengineering Chongqing University of Technology  Chongqing 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