{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:20:53Z","timestamp":1791426053706,"version":"4.3.3"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2003,8,18]],"date-time":"2003-08-18T00:00:00Z","timestamp":1061164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent studies have suggested that <jats:italic>ex vivo<\/jats:italic> expansion of autologous hematopoietic cells could be a therapy of choice for the treatment of bone marrow failure. We investigated the potential of a combined infusion of autologous <jats:italic>ex vivo<\/jats:italic> expanded hematopoietic cells with mesenchymal (MSCs) for the treatment of multi\u2010organ failure syndrome following irradiation in a non\u2010human primate model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hematopoietic cells and MSCs were expanded from bone marrow aspirates. MSCs were transduced with the gene encoding for the green fluorescent protein (e\u2010GFP), in order to track them following infusion. Twelve animals were studied. Nine animals received total\u2010body irradiation at 8 Gy from a neutron\/gamma source thus resulting in heterogeneous exposure; three animals were sham\u2010irradiated. The animals were treated with expanded hematopoietic stem cells and MSCs, expanded hematopoietic stem cells alone, or MSCs alone. Unmanipulated bone marrow cell transplants were used as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Depending on the neutron\/gamma ratio, an acute radiation sickness of varying severity but of similar nature resulted. GFP\u2010labeled cells were found in the injured muscle, skin, bone marrow and gut of the treated animals via PCR up to 82 days post\u2010infusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first evidence of expanded MSCs homing in numerous tissues following a severe multi\u2010organ injury in primates. Localization of the transduced MSCs correlated to the severity and geometry of irradiation. A repair process was observed in various tissues. The plasticity potential of the MSCs and their contribution to the repair process <jats:italic>in vivo<\/jats:italic> remains to be studied. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.452","type":"journal-article","created":{"date-parts":[[2003,12,3]],"date-time":"2003-12-03T08:00:38Z","timestamp":1070438438000},"page":"1028-1038","source":"Crossref","is-referenced-by-count":380,"title":["Mesenchymal stem cells home to injured tissues when co\u2010infused with hematopoietic cells to treat a radiation\u2010induced multi\u2010organ failure syndrome"],"prefix":"10.1002","volume":"5","author":[{"given":"Alain","family":"Chapel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean 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