{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:55:02Z","timestamp":1777589702633,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T00:00:00Z","timestamp":1080604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The tissue\u2010specific expression of an exogenous gene, under the influence of a tissue\u2010specific promoter, has been examined in the past with pro\u2010nuclear injections of the transgene and the development of transgenic mouse models. \u2018Adult transgenics\u2019 is possible with the acute expression of an exogenous gene that is administered to adult animals, providing the transgene can be effectively delivered to distant sites following an intravenous administration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The organ specificity of exogenous gene expression in adult mice was examined with a bacterial \u03b2\u2010galactosidase (LacZ) expression plasmid under the influence of the bovine rhodopsin gene promoter. The 8\u2010kb plasmid DNA was delivered to organs following an intravenous administration with the pegylated immunoliposome (PIL) non\u2010viral gene transfer technology. The PIL carrying the gene was targeted to organs with the rat 8D3 monoclonal antibody (MAb) to the mouse transferrin receptor (TfR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The rhodopsin\/\u03b2\u2010galactosidase gene was expressed widely in both the eye and the brain of adult mice, but was not expressed in peripheral tissues, including liver, spleen, lung, or heart. Ocular expression included the retinal\u2010pigmented epithelium, the iris, and ciliary body, and brain expression was observed in neuronal structures throughout the cerebrum and cerebellum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The expression of trans\u2010genes in adult animals is possible with the PIL non\u2010viral gene transfer method. The opsin promoter enables tissue\u2010specific gene expression in the eye, as well as the brain of adult mice, whereas gene expression in peripheral tissues, such as liver or spleen, is not observed. 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