{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:15:07Z","timestamp":1773281707618,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T00:00:00Z","timestamp":1080604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In mouse models of retinopathy of prematurity (ROP) inhibitors of vascular endothelial growth factor (VEGF) functions administered systemically completely block retinal neovascularization. In contrast, selective ocular VEGF depletion has achieved an approx. 50% inhibition of retinal neovascular growth. It is unclear whether a more complete inhibition of new blood vessel development can be obtained with an anti\u2010VEGF therapy localized to the eye. Therefore, the objective of the present study was to determine the effect of local anti\u2010VEGF therapy in a different animal model which closely mimics human ROP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats were exposed to alternating cycles of high and low levels of oxygen for 14 days immediately after birth; thereafter, they were intravitreally injected with an adenoviral vector expressing a secreted form of the VEGF receptor <jats:italic>flt\u20101<\/jats:italic> (Ad.<jats:italic>sflt<\/jats:italic>), which acts by sequestering VEGF. Contralateral eyes were injected with the control vector carrying the reporter gene expressing \u03b2\u2010galactosidase (Ad.\u03b2<jats:italic>Gal<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At the peak of retinal neovascular growth, i.e. post\u2010natal day 21 (P21), we observed up to 97.5% decrease in retinal neovascularization in animals injected with Ad.<jats:italic>sflt<\/jats:italic>. At the end of observation (P28), no significant difference in retinal vessel number was detected in both oxygen\u2010injured and normoxic Ad.<jats:italic>sflt<\/jats:italic>\u2010treated retinas compared with untreated or Ad.\u03b2<jats:italic>Gal<\/jats:italic>\u2010treated retinas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Adenoviral\u2010mediated sflt\u20101 gene transfer induces a near\u2010complete inhibition of ischemia\u2010induced retinal neovascularization in rats without affecting pre\u2010existing retinal vessels. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.586","type":"journal-article","created":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T11:53:25Z","timestamp":1080647605000},"page":"992-1002","source":"Crossref","is-referenced-by-count":40,"title":["Marked inhibition of retinal neovascularization in rats following soluble\u2010flt\u20101 gene transfer"],"prefix":"10.1002","volume":"6","author":[{"given":"Rossella","family":"Rota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Riccioni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Zaccarini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Lamartina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna 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