{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:21:44Z","timestamp":1792002104200,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Postmenopausal osteoporosis (PMO) is mainly concerned with the imbalance of bone resorption and bone formation. Icariin (ICA) plays a vital role in bone protection. This study investigated the mechanism of ICA in PMO rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The rats were treated with ovariectomy (OVX) and ICA. Bone structure parameters were measured by Micro\u2010CT. BMSCs were obtained from normal rats, OVX rats, and ICA\u2010treated rats. BMSCs were infected with SOST overexpression lentivirus, and TWS119, an activator of Wnt pathway, was introduced for joint experiment. The binding of ER\u03b1 to SOST promoter was verified. OVX\/ICA rats were injected with DNA methyltransferase inhibitor 5\u2010Aza\u2010dC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>ICA increased bone mass and decreased bone marrow fat content in OVX rats. ICA facilitated osteogenic differentiation and repressed adipogenic differentiation of BMSCs. Overexpressing SOST antagonized the effect of ICA, whereas TWS119 rescued the effect of overexpressing SOST. ICA reduced SOST expression by attenuating the effect of ER\u03b1. Methylation of SOST inhibited ER\u03b1 binding to SOST promoter. In\u00a0vivo experiments confirmed that ICA improved bone mass and reduced bone marrow fat content by enhancing SOST methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, ICA upregulated SOST methylation and inhibited the binding of ER\u03b1 to SOST promoter, thereby promoting osteogenic differentiation and repressing adipogenic differentiation of BMSCs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.70010","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T23:20:26Z","timestamp":1737588026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Icariin Facilitates Osteogenic Differentiation and Suppresses Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Enhancing SOST Methylation in Postmenopausal Osteoporosis"],"prefix":"10.1002","volume":"27","author":[{"given":"Xu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen City Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Orthopedics and Traumatology Orthopaedic Research Institute  Guangzhou City China"},{"name":"Department of Spinal Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen City Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqing","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen City Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuxin","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen City Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10354-015-0417-y"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1384629"},{"key":"e_1_2_9_4_1","first-page":"7232","article-title":"LncRNA HOTAIR Inhibited Osteogenic Differentiation of BMSCs by Regulating Wnt\/Beta\u2010Catenin Pathway","volume":"23","author":"Shen J. 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