{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T07:10:09Z","timestamp":1747379409718,"version":"3.40.5"},"reference-count":77,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Idiopathic pulmonary fibrosis (IPF) is a lethal disease with an unknown etiology and complex pathophysiology that are not fully understood. The disease involves intricate cellular interplay, particularly among various immune cells. Currently, there is no treatment capable of reversing the fibrotic process or aiding lung regeneration. Hepatocyte growth factor (HGF) has demonstrated antifibrotic properties, whereas the adoptive transfer of modified T cells is a well\u2010established treatment for various malignancies. We aimed to understand the dynamics of T cells in the progression of lung fibrosis and to study the therapeutic benefit of adoptive T cell transfer in a bleomycin\u2010injured mouse lung (BLM) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>T cells were isolated from the spleen of na\u00efve mice and transfected in\u00a0vitro with mouse HGF plasmid and were administered intratracheally to the mice lungs 7\u2009days post\u2010bleomycin injury to the lung. Lung tissue and bronchoalveolar lavage were collected and analyzed using flow cytometry, histology, qRT\u2010PCR, ELISA, and hydroxyproline assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our findings demonstrate the successful T cell therapy of bleomycin\u2010induced lung injury through the adoptive transfer of HGF\u2010transfected T cells in mice. This treatment resulted in decreased collagen deposition and a balancing of immune cell exhaustion and cytokine homeostasis compared with untreated controls. In\u00a0vitro testing showed enhanced apoptosis in myofibroblasts induced by HGF\u2010overexpressing T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken together, our data highlight the great potential of adoptive T cell transfer as an emerging therapy to counteract lung fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.70018","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T23:17:12Z","timestamp":1743463032000},"page":"1-16","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adoptive Transfer of T Cells as a Potential Therapeutic Approach in the Bleomycin\u2010Injured Mouse Lung"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0289-3881","authenticated-orcid":false,"given":"Seyran","family":"Mutlu","sequence":"first","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"},{"name":"Graduate School for Cellular and Biomedical Sciences University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kleanthis","family":"Fytianos","sequence":"additional","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Ferri\u00e9","sequence":"additional","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Scalise","sequence":"additional","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Mykoniati","sequence":"additional","affiliation":[{"name":"Unisant\u00e9  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amiq","family":"Gazdhar","sequence":"additional","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3374-0414","authenticated-orcid":false,"given":"Fabian","family":"Blank","sequence":"additional","affiliation":[{"name":"Department for Pulmonary Medicine, Allergology and Clinical Immunology, Inselspital, Bern University Hospital University of Bern  Bern Switzerland"},{"name":"Lung Precision Medicine (LPM), Department for BioMedical Research (DBMR) University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMRA003200"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RMED.2014.03.015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/THX.2010.148031"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12890\u2010021\u201001783\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0150383"},{"key":"e_1_2_10_7_1","unstructured":"J.BehrandD.Nowak \u201cTobacco Smoke and Respiratory Disease [Online] \u201d 14 Feb. 2025 https:\/\/www.researchgate.net\/publication\/251883915."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(11)60052\u20104"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/CRJ.13466"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMED.2018.00043"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/JEM.20110551"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2022.820347\/BIBTEX"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/AJPLUNG.00565.2016"},{"key":"e_1_2_10_14_1","unstructured":"M. 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